CBM UniTrap Project

Gene ENSMUSG00000053931 (Cnn3)

Chromosomal location	Chr 3: 121129452 - 121160867 (+)
Description	calponin 3, acidic Gene [Source:MGI Symbol;Acc:MGI:1919244]
RefSeq_dna	NM_028044
RefSeq_peptide	NP_082320.1
MGI	MGI:1919244
Uniprot/SWISSPROT	Q9DAW9
Uniprot/SPTREMBL	Q99JN5
Human Ortholog	ENSG00000117519 (CNN3)
Omim	not available

UniTrap UNI1457

Vector Insertion	Chr 3: 121129747 - 121152866
Public Clones	XS0351 (sanger) (sanger) CD0179 (sanger) CD0488 (sanger) RRS022 (baygenomics) H003E07 (ggtc) P140A03 (ggtc) D117A11 (ggtc) IST14584H4 (tigm) IST14428F5 (tigm) IST14262D7 (tigm) IST11406G5 (tigm) IST10037C11 (tigm) IST14669A5 (tigm) IST15055D12 (tigm) IST14965E5 (tigm)
Private Clones	OST448804 (lexicon) OST415709 (lexicon) OST401902 (lexicon) OST396574 (lexicon) OST282971 (lexicon) OST278196 (lexicon) OST273721 (lexicon) OST248295 (lexicon) OST201904 (lexicon) OST188464 (lexicon) OST177463 (lexicon) OST175092 (lexicon) OST168030 (lexicon) OST151676 (lexicon) OST129369 (lexicon) OST115700 (lexicon) OST99691 (lexicon) OST45833 (lexicon) OST43124 (lexicon) OST35372 (lexicon) OST34731 (lexicon)
Severity of mutation (?)	Insertion after 6% of polypeptide chain
Proposed experimental design for vector insertion validation (?)

UniTrap UNI17292

Vector Insertion	Chr 3: 121152989 - 121153209
Public Clones	not available
Private Clones	OST445798 (lexicon) OST419273 (lexicon) OST242387 (lexicon) OST221948 (lexicon) OST213524 (lexicon) OST131288 (lexicon) OST78861 (lexicon) OST32716 (lexicon) OST8147 (lexicon)
Severity of mutation (?)	Insertion after 18% of polypeptide chain
Proposed experimental design for vector insertion validation (?)

UniTrap UNI22035

Vector Insertion	Chr 3: 121153277 - 121154300
Public Clones	not available
Private Clones	OST311051 (lexicon) OST237169 (lexicon) OST79101 (lexicon) OST65351 (lexicon)
Severity of mutation (?)	Insertion after 25% of polypeptide chain
Proposed experimental design for vector insertion validation (?)

UniTrap UNI25248

Vector Insertion	Chr 3: 121154439 - 121154800
Public Clones	not available
Private Clones	OST190357 (lexicon)
Severity of mutation (?)	Insertion after 39% of polypeptide chain
Proposed experimental design for vector insertion validation (?)

Show all transcripts and translations:

Transcript ENSMUST00000029773

Sequence

GCGGAGCGGGCAGCGAGCAGGGCAGCCGGTCCCAGTCCAGAGCGACCCGGAGCCTCCGCGGGACTCGAGTCCTAGCGAACCTCGAAGCATCATCCGCGTC

CGTCTGCGGCGTTCGGGCTTCTCCGGCGCCGCGCAGGGGAGCGAGCTCGTGCATCACCCGCGCGGCCGCCGCTGGGGCTAAGAGCAGGGACACCGAGGGT

GACTGACCGCTAGAACTCTTCCAGTCGTCCAAACAGCCATGACCCACTTCAACAAGGGCCCTTCCTACGGGCTCTCCGCCGAAGTTAAGAACAAG - UN

I1457 - ATTGCATCCAAGTATGACCAGCAGGCCGAGGAAGATCTGCGCAACTGGATAGAAGAGGTGACAGGCCTAGGCATTGGCACCAACTTCCAGCT

GGGGCTGAAGGACGGCATCATATTGTGCGA - UNI17292 - ACTCATAAACAAGCTACAGCCAGGCTCTGTGAAGAAAGTCAACGAATCCTCACTAA

ATTGGCCCCAG - UNI22035 - TTGGAGAATATCGGCAACTTCATTAAAGCTATCCAGGCTTACGGTATGAAGCCCCATGATATATTTGAAGCAAAC

GACCTCTTTGAGAATGGCAACATGACCCAGGTTCAGACGACGCTGGTGGCTCTAGCAGGTCTG - UNI25248 - GCGAAAACAAAAGGATTCCATAC

AACCATTGACATTGGCGTTAAGTATGCAGAAAAACAAACAAGACGTTTTGATGAAGGCAAATTAAAGGCTGGCCAGAGTGTAATTGGTTTACAG ATGGG

TACCAACAAATGTGCCAGCCAGGCGGGCATGACAGCCTATGGGACTCGGAGGCATCTTTATGATCCCAAGATGCAGACGGACAAACCCTTTGACCAGACC

ACGATTAGCCTGCAGATGGGCACCAACAAAGGGGCCAGCCAG GCTGGGATGTTAGCACCGGGCACCAGAAGAGACATCTATGACCAGAAGCTGACATTA

CAGCCAGTGGACAACTCGACCATTTCTCTACAGATGGGCACCAACAAAGTTGCTTCCCAGAAAGGAATGAGCGTGTATGGGCTTGGGCGGCAAGTATATG

ACCCCAAGTACTGTGCCGCACCCACAGAACCTGTCATTCACAACGGAAGCCAGGGCACGGGCACCAATGGGTCGGAAATCAGTGATAGCGATTATCAGGC

AGAATACCCCGATGAATATCATGGCGAGTACCCAGACGACTACCCTCGGGAGTACCAGTATGGCGACGACCAGGGCATTGATTATTAGAGTCACACACAG

GAGCGCAGTATTTAGTCCATTGTTTTATCCAGTGAGACCCAAGCTAGCCTTGAATAATTCTTCTCTCGTCTTCCTGAAACACTATTATGCTTGTTGTACC

TTTAAAGTATGCCTTATGTACATTCCTTTCTCCTTTTCCTGCCTCCTCCCTAAATAGCTGCCTTCTAGTGCTGTAGCAAGGGAGCCCTACTGCATAGCCA

GTAACTCGCGTCTGTACCATGGAAAGGGCGGAACGATTCTCCAGGACAGCCAGCTCTTTCGTTGAAGATCTATCTATGCATTTTTTTACACTTACACATA

AACTGGTATTTTCGAACAATAGGAAACTATTTTTTCTCCTTTTTTTACAGTTTTAGTACGTATCTGGCTTGTATGTGGAAGACTAAAAAGTTGATTTGCT

AAATGTGGTCTTTGCCAACTAAAATCTGAGATGCAGCTTTAGACCCTGACACGTGGATGTTCTTCTGCAGTCTTGTCTGCTAAGTTTTAAATAAAGTCAT

GATCAGTGTGC

Translation

MTHFNKGPSYGLSAEVKNK - UNI1457 - IASKYDQQAEEDLRNWIEEVTGLGIGTNFQLGLKDGIILCE - UNI17292 - ELINKLQPGSVKK

VNESSLNWPQ - UNI22035 - LENIGNFIKAIQAYGMKPHDIFEANDLFENGNMTQVQTTLVALAGL - UNI25248 - AKTKGFHTTIDIGVKY

AEKQTRRFDEGKLKAGQSVIGLQ MGTNKCASQAGMTAYGTRRHLYDPKMQTDKPFDQTTISLQMGTNKGASQ AGMLAPGTRRDIYDQKLTLQPVDNST

ISLQMGTNKVASQKGMSVYGLGRQVYDPKYCAAPTEPVIHNGSQGTGTNGSEISDSDYQAEYPDEYHGEYPDDYPREYQYGDDQGIDY

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