Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI10117
Trapped Gene
Dcbld1 (ENSMUSG00000019891)
Vector Insertion
Chr 10: 52024527 - 52031205
Public Clones XST043 (baygenomics)
Private Clones not available
Severity of mutation (?) Insertion after 37% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000490106 (Chr10:52024393..52024526 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000490106 (Chr10:52024393..52024526 +)
Downstram Exon
ENSMUSE00000487939 (Chr10:52031206..52031246 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000502943 Chr10:51953425..51953641 No primer for this exon
upstream ENSMUSE00000466026 Chr10:51981533..51981745 No primer for this exon
upstream ENSMUSE00000302877 Chr10:52003909..52004043 No primer for this exon
upstream ENSMUSE00000302871 Chr10:52006154..52006205 No primer for this exon
upstream ENSMUSE00000302865 Chr10:52010657..52010729 No primer for this exon
upstream ENSMUSE00000490106 Chr10:52024393..52024526 No primer for this exon

*** Putative Vector Insertion (Chr 10: 52024527 - 52031205) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000487939 Chr10:52031206..52031246 No primer for this exon
downstream ENSMUSE00000098830 Chr10:52032620..52032707 No primer for this exon
downstream ENSMUSE00000098836 Chr10:52034161..52034210 No primer for this exon
downstream ENSMUSE00000098834 Chr10:52036838..52036957 No primer for this exon
downstream ENSMUSE00000447452 Chr10:52039282..52040081 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TGGCTTGTGTTCTGAGAGGA Chr10:52024537..52024557 59.54 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TGGCTTGTGTTCTGAGAGGA Chr10:52024537..52024557 59.54 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000019891