Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI10230
Trapped Gene
Epha2 (ENSMUSG00000006445)
Vector Insertion
Chr 4: 140857350 - 140862428
Public Clones RST009 (baygenomics) GST015 (baygenomics) P086D08 (ggtc) D021A11 (ggtc)
E037B11 (ggtc) G061C12 (ggtc) D039G03 (ggtc) G062E11 (ggtc) P136E01 (ggtc)
(ggtc) D039G03 (ggtc) D044D11 (ggtc) P063B05 (ggtc) D021A11 (ggtc)
D044D11 (ggtc) P128C07 (ggtc) P056C09 (ggtc) D039G03 (ggtc) IST14816F4 (tigm)
Private Clones OST208550 (lexicon)
Severity of mutation (?) Insertion after 3% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000309437 (Chr4:140857155..140857349 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000309437 (Chr4:140857155..140857349 +)
Downstram Exon
ENSMUSE00000309430 (Chr4:140862429..140862496 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000309437 Chr4:140857155..140857349 No primer for this exon

*** Putative Vector Insertion (Chr 4: 140857350 - 140862428) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000309430 Chr4:140862429..140862496 No primer for this exon
downstream ENSMUSE00000309419 Chr4:140864320..140864995 No primer for this exon
downstream ENSMUSE00000309411 Chr4:140872502..140872657 No primer for this exon
downstream ENSMUSE00000309404 Chr4:140872786..140873118 No primer for this exon
downstream ENSMUSE00000309398 Chr4:140874397..140874512 No primer for this exon
downstream ENSMUSE00000309394 Chr4:140874838..140874991 No primer for this exon
downstream ENSMUSE00000309389 Chr4:140876409..140876508 No primer for this exon
downstream ENSMUSE00000309380 Chr4:140877132..140877187 No primer for this exon
downstream ENSMUSE00000309369 Chr4:140877380..140877505 No primer for this exon
downstream ENSMUSE00000381679 Chr4:140877617..140877805 No primer for this exon
downstream ENSMUSE00000350172 Chr4:140877978..140878039 No primer for this exon
downstream ENSMUSE00000309345 Chr4:140878118..140878327 No primer for this exon
downstream ENSMUSE00000524376 Chr4:140878501..140878650 No primer for this exon
downstream ENSMUSE00000183465 Chr4:140879328..140879521 No primer for this exon
downstream ENSMUSE00000309324 Chr4:140880147..140880302 No primer for this exon
downstream ENSMUSE00000360227 Chr4:140884328..140885293 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 AGGGACTCCGGGACATAATC Chr4:140857386..140857406 60.15 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GACACGTGACTGGGAAAACC Chr4:140857397..140857417 60.41 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000006445