Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI10237
Trapped Gene
Fut8 (ENSMUSG00000021065)
Vector Insertion
Chr 12: 78513937 - 78549459
Public Clones GST093 (baygenomics)
Private Clones not available
Severity of mutation (?) Insertion after 49% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000114289 (Chr12:78513699..78513936 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000114289 (Chr12:78513699..78513936 +)
Downstram Exon
ENSMUSE00000114293 (Chr12:78549460..78549706 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000531291 Chr12:78340052..78340240 No primer for this exon
upstream ENSMUSE00000453763 Chr12:78378348..78378442 No primer for this exon
upstream ENSMUSE00000453723 Chr12:78424832..78424924 No primer for this exon
upstream ENSMUSE00000453743 Chr12:78432883..78433316 No primer for this exon
upstream ENSMUSE00000390365 Chr12:78465956..78466071 No primer for this exon
upstream ENSMUSE00000114291 Chr12:78466184..78466346 No primer for this exon
upstream ENSMUSE00000395439 Chr12:78494710..78494824 No primer for this exon
upstream ENSMUSE00000114289 Chr12:78513699..78513936 No primer for this exon

*** Putative Vector Insertion (Chr 12: 78513937 - 78549459) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000114293 Chr12:78549460..78549706 No primer for this exon
downstream ENSMUSE00000114292 Chr12:78551087..78551263 No primer for this exon
downstream ENSMUSE00000114294 Chr12:78565047..78565197 No primer for this exon
downstream ENSMUSE00000531290 Chr12:78575986..78577325 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 ATAATCGCCTTGCAGCACAT Chr12:78534987..78535007 60.64 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CAGACTAGCAGCGTGACTGG Chr12:78534977..78534997 59.79 60 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000021065