Tools for the biologist enabling optimized use of gene trap clones
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UniTrap UNI10378
Trapped Gene 
Fn1 (ENSMUSG00000026193)
Vector Insertion 
Chr 1: 71637467 - 71639644
Public Clones Ex180 (baygenomics)
Private Clones not available
Severity of mutation (?) Insertion after 94% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000629675 (Chr1:71639645..71639809 -)		 Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
TTGACCCTTACACGGTTTCC Chr1:71639751..71639770 59.83 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000629675 (Chr1:71639645..71639809 -)		 Downstram Exon
ENSMUSE00000629674 (Chr1:71637341..71637466 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
TTGACCCTTACACGGTTTCC Chr1:71639751..71639770 59.83 50 CCCACTTCTCTCCGATCTTG Chr1:71637397..71637416 59.8 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000544656 Chr1:71699406..71699746 CTCCTCCCATCCACTCAAGA Chr1:71699556..71699575 60.19 55
upstream ENSMUSE00000629712 Chr1:71698095..71698223 GGGTTTTAACTGCGAGAGCA Chr1:71698104..71698123 60.39 50
upstream ENSMUSE00000629711 Chr1:71697136..71697273 ATGAGCGCCCTAAAGATTCC Chr1:71697197..71697216 60.55 50
upstream ENSMUSE00000629710 Chr1:71695768..71695899 AAGATTGGCGACAAGTGGAG Chr1:71695851..71695870 60.26 50
upstream ENSMUSE00000629709 Chr1:71694583..71694720 CAATGGACGCATCACCTGTA Chr1:71694592..71694611 60.54 50
upstream ENSMUSE00000629708 Chr1:71692121..71692276 AAGGACAACCGAGGAAACCT Chr1:71692198..71692217 59.97 50
upstream ENSMUSE00000629707 Chr1:71689534..71689725 GAAGTCGCAAGGAAACAAGC Chr1:71689582..71689601 60 50
upstream ENSMUSE00000629706 Chr1:71688526..71688705 TCCTCCCGTTCACCTACAAC Chr1:71688641..71688660 59.97 55
upstream ENSMUSE00000629705 Chr1:71687726..71687902 AACCGGAATTACACCGACTG Chr1:71687818..71687837 59.85 50
upstream ENSMUSE00000629704 Chr1:71686866..71687018 GTATCGCATTGGGGATCAGT Chr1:71686962..71686981 59.78 50
upstream ENSMUSE00000629703 Chr1:71685031..71685159 ACGAGGAGGGACATATGCTG Chr1:71685083..71685102 60.1 55
upstream ENSMUSE00000629702 Chr1:71683826..71683969 GACCCGGACATTTTACCAGA Chr1:71683931..71683950 59.79 50
upstream ENSMUSE00000629701 Chr1:71678615..71678736 GAGCCTTCACACATCACCAA Chr1:71678637..71678656 59.68 50
upstream ENSMUSE00000629700 Chr1:71676240..71676417 CAGAGAAGTGACACGCTTCG Chr1:71676273..71676292 59.77 55
upstream ENSMUSE00000629699 Chr1:71675890..71676066 TGTGGCCACTTCTGAATCTG Chr1:71676007..71676026 59.83 50
upstream ENSMUSE00000629698 Chr1:71674982..71675110 TCCGTGAACATTCCTGACCT Chr1:71675071..71675090 60.51 50
upstream ENSMUSE00000629697 Chr1:71674631..71674720 TAGACCCCAGGCACCTATCA Chr1:71674634..71674653 60.47 55
upstream ENSMUSE00000629696 Chr1:71673737..71673931 AGAGCACACCCGTTTTCATC Chr1:71673768..71673787 60.12 50
upstream ENSMUSE00000629695 Chr1:71672548..71672820 CCGACGGACCTACAGTTTGT Chr1:71672787..71672806 60.03 55
upstream ENSMUSE00000629694 Chr1:71670652..71670918 TGGGTCTGAGTACACCGTGA Chr1:71670712..71670731 60.15 55
upstream ENSMUSE00000629693 Chr1:71667299..71667393 CAACACCGAGGTGACAGAGA Chr1:71667346..71667365 59.86 55
upstream ENSMUSE00000629692 Chr1:71665958..71666126 ATGCACCGATTGTCAACAGA Chr1:71665968..71665987 60.12 45
upstream ENSMUSE00000629691 Chr1:71665153..71665239 CTGTCTCCACCGACCAACTT Chr1:71665218..71665237 60.15 55
upstream ENSMUSE00000629690 Chr1:71661934..71662125 TGCCCCTATCTCTGATACCG Chr1:71661943..71661962 60.05 55
upstream ENSMUSE00000544622 Chr1:71660337..71660609 ACCCCGCTAAACTCTTCCAC Chr1:71660525..71660544 60.5 55
upstream ENSMUSE00000629689 Chr1:71659042..71659224 GACAATGCCGTGGTCCTAAC Chr1:71659044..71659063 60.38 55
upstream ENSMUSE00000629688 Chr1:71657503..71657592 CGAACAACATGAGAGCATCC Chr1:71657527..71657546 59.24 50
upstream ENSMUSE00000629687 Chr1:71655987..71656256 TATCACCGCCAACTCATTCA Chr1:71656203..71656222 60.07 45
upstream ENSMUSE00000629686 Chr1:71654478..71654594 CTGCTCATCAGTTGGGAACC Chr1:71654525..71654544 60.66 55
upstream ENSMUSE00000629685 Chr1:71653277..71653441 GCAAGCCAGTTTCCATCAAT Chr1:71653287..71653306 60.08 45
upstream ENSMUSE00000629684 Chr1:71652459..71652614 GACCACCACTCCCAAAAATG Chr1:71652495..71652514 60.21 50
upstream ENSMUSE00000544614 Chr1:71651544..71651657 GGTTCAAACTGCAGTGACCA Chr1:71651544..71651563 59.73 50
upstream ENSMUSE00000377635 Chr1:71650234..71650503 TGGATGTCGATTCCATCAAA Chr1:71650448..71650467 59.86 40
upstream ENSMUSE00000629683 Chr1:71648884..71649071 TGGATAGCACCCAGTGTTCA Chr1:71648990..71649009 60.11 50
upstream ENSMUSE00000629682 Chr1:71647775..71647862 CAAGGACACACTGACAAGCAG Chr1:71647807..71647827 59.52 52.38
upstream ENSMUSE00000629681 Chr1:71646864..71647040 GTGTGACGGACGCTACAGAG Chr1:71646997..71647016 59.49 60
upstream ENSMUSE00000629680 Chr1:71646177..71646266 GCCAGGCACTGACTACAAGA Chr1:71646240..71646259 59.04 55
upstream ENSMUSE00000629679 Chr1:71645789..71645968 GATCCCCTCCCAGAGAAGTG Chr1:71645832..71645851 60.99 60
upstream ENSMUSE00000629678 Chr1:71644948..71645037 CCAGGAACCGAGTACACCAT Chr1:71645010..71645029 59.84 55
upstream ENSMUSE00000629671 Chr1:71643855..71644246 CAAGACCATACCTGCCGAAT Chr1:71644059..71644078 59.96 50
upstream ENSMUSE00000629677 Chr1:71643855..71644321 GGGTATGACACCGAAAATGG Chr1:71644198..71644217 60.05 50
upstream ENSMUSE00000629676 Chr1:71642238..71642376 ACAAGGTTCGGGAAGAGGTT Chr1:71642257..71642276 59.97 50
upstream ENSMUSE00000629675 Chr1:71639645..71639809 TTGACCCTTACACGGTTTCC Chr1:71639751..71639770 59.83 50

*** Putative Vector Insertion (Chr 1: 71637467 - 71639644) ***
  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000629674 Chr1:71637341..71637466 CCCACTTCTCTCCGATCTTG Chr1:71637397..71637416 59.8 55
downstream ENSMUSE00000629673 Chr1:71636672..71636778 TCGTCATAGCACGTTGCTTC Chr1:71636735..71636754 60.02 50
downstream ENSMUSE00000629672 Chr1:71633404..71633517 CAGGTCTACGGCAGTTGTCA Chr1:71633465..71633484 59.9 55
downstream ENSMUSE00000544580 Chr1:71632094..71632857 ATCTAGCGGCATGAAGCACT Chr1:71632797..71632816 60.01 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CACCCTCTCACTCCTCCATC Chr1:71639604..71639624 59.64 60 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CACCCTCTCACTCCTCCATC Chr1:71639604..71639624 59.64 60 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000026193