Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI10495
Trapped Gene
Csrp2 (ENSMUSG00000020186)
Vector Insertion
Chr 10: 110369124 - 110372203
Public Clones (sanger) D031E08 (ggtc) D031E08 (ggtc) 5SE305D12 (ggtc) (ggtc)
P112H12 (ggtc) D031E08 (ggtc) 3SE305D12 (ggtc) CMHD-GT_357C11-3 (cmhd) CMHD-GT_494D10-3 (cmhd)
PST2102-NR (escells) IST11686E12 (tigm) IST11200F9 (tigm) IST12275C3 (tigm)
IST11385G5 (tigm) IST11644H2 (tigm)
Private Clones OST469862 (lexicon) OST465353 (lexicon) OST462957 (lexicon) OST444446 (lexicon)
OST356467 (lexicon) OST305381 (lexicon) OST300052 (lexicon) OST253674 (lexicon)
OST241314 (lexicon) OST231445 (lexicon) OST197657 (lexicon) OST172107 (lexicon)
OST166093 (lexicon) OST166024 (lexicon) OST134731 (lexicon) OST96481 (lexicon)
OST67912 (lexicon) OST67099 (lexicon) OST61893 (lexicon) OST56844 (lexicon)
OST37633 (lexicon) OST37541 (lexicon) OST35474 (lexicon) OST30701 (lexicon)
OST30606 (lexicon) OST30269 (lexicon)
Severity of mutation (?) Insertion after 19% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000101586 (Chr10:110369011..110369123 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000101586 (Chr10:110369011..110369123 +)
Downstram Exon
ENSMUSE00000101593 (Chr10:110372204..110372372 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000369473 Chr10:110357235..110357310 No primer for this exon
upstream ENSMUSE00000101586 Chr10:110369011..110369123 No primer for this exon

*** Putative Vector Insertion (Chr 10: 110369124 - 110372203) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000101593 Chr10:110372204..110372372 No primer for this exon
downstream ENSMUSE00000101596 Chr10:110374793..110374922 No primer for this exon
downstream ENSMUSE00000573921 Chr10:110375693..110375786 No primer for this exon
downstream ENSMUSE00000438184 Chr10:110376267..110376678 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GTCATTTGGAGGCACCTCTG Chr10:110369076..110369096 60.66 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GTCATTTGGAGGCACCTCTG Chr10:110369076..110369096 60.66 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000020186