Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI10633
Trapped Gene
Creld1 (ENSMUSG00000030284)
Vector Insertion
Chr 6: 113434071 - 113434488
Public Clones M117A03 (ggtc)
Private Clones not available
Severity of mutation (?) Insertion after 14% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000224405 (Chr6:113433554..113434070 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
GCACTGGTGGACAACTTCAA Chr6:113434047..113434066 59.73 50 TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000224405 (Chr6:113433554..113434070 +)
Downstram Exon
ENSMUSE00000197059 (Chr6:113434489..113434571 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
GCACTGGTGGACAACTTCAA Chr6:113434047..113434066 59.73 50 AAGTTGTCCCGGATGGTTCT Chr6:113434520..113434539 60.75 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000224405 Chr6:113433554..113434070 GCACTGGTGGACAACTTCAA Chr6:113434047..113434066 59.73 50

*** Putative Vector Insertion (Chr 6: 113434071 - 113434488) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000197059 Chr6:113434489..113434571 AAGTTGTCCCGGATGGTTCT Chr6:113434520..113434539 60.75 50
downstream ENSMUSE00000197055 Chr6:113438065..113438175 AACCACCAGTTTTCCACCAG Chr6:113438172..113438191 59.86 50
downstream ENSMUSE00000197051 Chr6:113438473..113438564 CTTCAGGGAATCGGAACAGA Chr6:113438527..113438546 60.19 50
downstream ENSMUSE00000197061 Chr6:113439428..113439604 AAGTAGCCAAGGCCACACTG Chr6:113439569..113439588 60.31 55
downstream ENSMUSE00000197065 Chr6:113439689..113439784 CACTGCAGACAATGGGATTC Chr6:113439749..113439768 59.09 50
downstream ENSMUSE00000197063 Chr6:113441647..113441730 GTGGCTTGCTCTGTACCACA Chr6:113441680..113441699 59.9 55
downstream ENSMUSE00000197052 Chr6:113441888..113441983 No primer for this exon
downstream ENSMUSE00000197050 Chr6:113442118..113442252 CCCTCCGTGTTTTCACACTT Chr6:113442184..113442203 60.01 50
downstream ENSMUSE00000377202 Chr6:113442681..113443333 TTTCATCCTCCGTCATCTCC Chr6:113442722..113442741 60.01 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TCAACAAGGTGGGTGCACTA Chr6:113434064..113434084 60.15 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TCAACAAGGTGGGTGCACTA Chr6:113434064..113434084 60.15 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000030284