Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI10712
Trapped Gene
Brca1 (ENSMUSG00000017146)
Vector Insertion
Chr 11: 101410435 - 101413082
Public Clones (sanger) (sanger) (sanger) (sanger) P025G05 (ggtc) E080C01 (ggtc)
H004D01 (ggtc) M122A01 (ggtc) M122A01 (ggtc) (ggtc) H004D01 (ggtc)
IST10084G10 (tigm) IST10828G6 (tigm) IST10828G6BBF1 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000333507 (Chr11:101413083..101413126 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000333507 (Chr11:101413083..101413126 -)
Downstram Exon
ENSMUSE00000672474 (Chr11:101410329..101410434 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000333507 Chr11:101413083..101413126 No primer for this exon

*** Putative Vector Insertion (Chr 11: 101410435 - 101413082) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000389811 Chr11:101410329..101410427 No primer for this exon
downstream ENSMUSE00000672474 Chr11:101410329..101410434 No primer for this exon
downstream ENSMUSE00000113036 Chr11:101401295..101401348 No primer for this exon
downstream ENSMUSE00000113047 Chr11:101396842..101396919 No primer for this exon
downstream ENSMUSE00000113052 Chr11:101395253..101395341 No primer for this exon
downstream ENSMUSE00000113045 Chr11:101393334..101393473 No primer for this exon
downstream ENSMUSE00000113044 Chr11:101392306..101392408 No primer for this exon
downstream ENSMUSE00000113039 Chr11:101391105..101391150 No primer for this exon
downstream ENSMUSE00000113037 Chr11:101389315..101389391 No primer for this exon
downstream ENSMUSE00000113042 Chr11:101384639..101387953 No primer for this exon
downstream ENSMUSE00000113048 Chr11:101383746..101383816 No primer for this exon
downstream ENSMUSE00000113043 Chr11:101378585..101378756 No primer for this exon
downstream ENSMUSE00000113040 Chr11:101374034..101374163 No primer for this exon
downstream ENSMUSE00000113046 Chr11:101371235..101371413 No primer for this exon
downstream ENSMUSE00000240223 Chr11:101369264..101369544 No primer for this exon
downstream ENSMUSE00000113055 Chr11:101366631..101366718 No primer for this exon
downstream ENSMUSE00000113054 Chr11:101363795..101363872 No primer for this exon
downstream ENSMUSE00000113049 Chr11:101363306..101363346 No primer for this exon
downstream ENSMUSE00000113033 Chr11:101359248..101359328 No primer for this exon
downstream ENSMUSE00000113053 Chr11:101354866..101354920 No primer for this exon
downstream ENSMUSE00000113035 Chr11:101353495..101353568 No primer for this exon
downstream ENSMUSE00000113051 Chr11:101353173..101353233 No primer for this exon
downstream ENSMUSE00000585847 Chr11:101350881..101351226 No primer for this exon
downstream ENSMUSE00000672450 Chr11:101350078..101351226 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GAGAAAAGGTGAGCGTGACTG Chr11:101413068..101413089 60.04 52.38 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000017146