Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI10817
Trapped Gene
Mat2a (ENSMUSG00000053907)
Vector Insertion
Chr 6: 72387112 - 72387364
Public Clones P122A08 (ggtc) P122A08 (ggtc)
Private Clones not available
Severity of mutation (?) Insertion after 19% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000429009 (Chr6:72387365..72387442 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
ATGCTGTCCTTGATGCACAC Chr6:72387399..72387418 59.71 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000429009 (Chr6:72387365..72387442 -)
Downstram Exon
ENSMUSE00000428558 (Chr6:72386989..72387111 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
ATGCTGTCCTTGATGCACAC Chr6:72387399..72387418 59.71 50 GGATGTAATTTCCCCAGCAA Chr6:72387040..72387059 59.76 45

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000429035 Chr6:72389340..72389550 AGGGGACGTTCCTCTTCACT Chr6:72389368..72389387 60.11 55
upstream ENSMUSE00000429009 Chr6:72387365..72387442 ATGCTGTCCTTGATGCACAC Chr6:72387399..72387418 59.71 50

*** Putative Vector Insertion (Chr 6: 72387112 - 72387364) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000428558 Chr6:72386989..72387111 GGATGTAATTTCCCCAGCAA Chr6:72387040..72387059 59.76 45
downstream ENSMUSE00000428552 Chr6:72386411..72386523 ACTGTTGTTCCAAGGCAACC Chr6:72386454..72386473 60.01 50
downstream ENSMUSE00000428543 Chr6:72386191..72386334 ATGTACCATTGCGGCGTAGT Chr6:72386201..72386220 60.42 50
downstream ENSMUSE00000428538 Chr6:72385805..72386023 TTGCAGGTACAACAGCCTTG Chr6:72385851..72385870 59.9 50
downstream ENSMUSE00000559585 Chr6:72385302..72385382 ATGATTTTTCGGCCAGTCAG Chr6:72385329..72385348 60.07 45
downstream ENSMUSE00000559584 Chr6:72385231..72385296 GAGCAGCATAAGCAGCTGAA Chr6:72385234..72385253 59.47 50
downstream ENSMUSE00000428529 Chr6:72385200..72385382 ATGATTTTTCGGCCAGTCAG Chr6:72385329..72385348 60.07 45
downstream ENSMUSE00000615638 Chr6:72384975..72385108 TGGAGATCGACAATGGATGA Chr6:72385044..72385063 60.01 45
downstream ENSMUSE00000615637 Chr6:72382793..72384389 CCAACAAGTCTGGGGAAAAA Chr6:72384233..72384252 59.94 45

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TCCTTGATGCACACCTTCAA Chr6:72387391..72387411 60.24 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TCCTTGATGCACACCTTCAA Chr6:72387391..72387411 60.24 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 ATGCTGTCCTTGATGCACAC Chr6:72387397..72387417 59.71 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 ATGCTGTCCTTGATGCACAC Chr6:72387397..72387417 59.71 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000053907