Tools for the biologist enabling optimized use of gene trap clones
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About
UniTrap UNI10860
Trapped Gene
Cox8a (
ENSMUSG00000035885
)
Vector Insertion
Chr 19: 7289975 - 7291888
Public Clones
(sanger)
(sanger)
G053F05 (ggtc)
P075H08 (ggtc)
G048E03 (ggtc)
CMHD-GT_535G10-3 (cmhd)
(cmhd)
CMHD-GT_410A4-3 (cmhd)
CMHD-GT_533G6-3 (cmhd)
CMHD-GT_427B9-3 (cmhd)
IST13053A10 (tigm)
Private Clones
OST386350 (lexicon)
OST378979 (lexicon)
OST364602 (lexicon)
OST233109 (lexicon)
OST43285 (lexicon)
OST37468 (lexicon)
OST22432 (lexicon)
OST1120 (lexicon)
Severity of mutation
(?)
Insertion after 55% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000346434 (Chr19:7291889..7292056 -)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
CTGACCTTGGACTGGTCCTC
Chr19:7292026..7292045
59.68
60
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000346434 (Chr19:7291889..7292056 -)
Downstram Exon
ENSMUSE00000239800 (Chr19:7289653..7289974 -)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
CTGACCTTGGACTGGTCCTC
Chr19:7292026..7292045
59.68
60
TCGAGAGGTCATGATTGCAG
Chr19:7289717..7289736
59.94
50
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000346434
Chr19:7291889..7292056
CTGACCTTGGACTGGTCCTC
Chr19:7292026..7292045
59.68
60
*** Putative Vector Insertion (Chr 19: 7289975 - 7291888) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000239800
Chr19:7289653..7289974
TCGAGAGGTCATGATTGCAG
Chr19:7289717..7289736
59.94
50
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
GTAATCGCCTTGCAGCACAT
Chr19:7291818..7291838
61.2
50
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
CTGTGGTAGCCTGCGTGACT
Chr19:7291830..7291850
61.47
60
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
PCR 3
GACTCCCTGACCTTGGACTG
Chr19:7292030..7292050
59.68
60
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 4
GACTCCCTGACCTTGGACTG
Chr19:7292030..7292050
59.68
60
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000035885