Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI10902
Trapped Gene
Tgif1 (ENSMUSG00000047407)
Vector Insertion
Chr 17: 71195739 - 71200211
Public Clones (sanger) (sanger) (sanger) (sanger) 3SD039C02 (ggtc) 5SP119G07 (ggtc)
3SD029G07 (ggtc) 3SD080F05 (ggtc) D037B02 (ggtc) 5SD006A11 (ggtc)
(ggtc) 3SD039B07 (ggtc) 3SP119G07 (ggtc) 5SE040A03 (ggtc) (ggtc)
P109A03 (ggtc) 5SP140F12 (ggtc) 3SD006A11 (ggtc) 5SD029G07 (ggtc)
3SD144C07 (ggtc) 5SD010D04 (ggtc) (ggtc) PST10520-NR (escells) IST14227G1 (tigm)
IST13698G4 (tigm) IST12478C5 (tigm) IST14630A5 (tigm) IST14958G9 (tigm)
IST12478C5 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000692627 (Chr17:71200212..71200550 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
TCCTAGAAACCCCAGCTTCA Chr17:71200254..71200273 59.81 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000692627 (Chr17:71200212..71200550 -)
Downstram Exon
ENSMUSE00000606362 (Chr17:71195512..71195738 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
TCCTAGAAACCCCAGCTTCA Chr17:71200254..71200273 59.81 50 AGAGGCTGCTGATGAGGAAA Chr17:71195634..71195653 60.1 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000710962 Chr17:71202788..71202886 CGCTCCGACTTCTTAACTGC Chr17:71202852..71202871 60.15 55
upstream ENSMUSE00000718089 Chr17:71200650..71201074 AAGGATGGGGGTGTCTTTTC Chr17:71200894..71200913 60.17 50
upstream ENSMUSE00000692627 Chr17:71200212..71200550 TCCTAGAAACCCCAGCTTCA Chr17:71200254..71200273 59.81 50

*** Putative Vector Insertion (Chr 17: 71195739 - 71200211) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000606362 Chr17:71195512..71195738 AGAGGCTGCTGATGAGGAAA Chr17:71195634..71195653 60.1 50
downstream ENSMUSE00000716636 Chr17:71195512..71195738 AGAGGCTGCTGATGAGGAAA Chr17:71195634..71195653 60.1 50
downstream ENSMUSE00000714388 Chr17:71193554..71194612 ATCCTGGTTGAGGTCTGGTG Chr17:71194096..71194115 59.96 55
downstream ENSMUSE00000606361 Chr17:71193552..71194612 ATCCTGGTTGAGGTCTGGTG Chr17:71194096..71194115 59.96 55
downstream ENSMUSE00000717035 Chr17:71193551..71194612 ATCCTGGTTGAGGTCTGGTG Chr17:71194096..71194115 59.96 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CAGTGCGGGACATGTTAATC Chr17:71200155..71200175 59 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 ATCAAAGGGCAGGCTTTAGG Chr17:71200200..71200220 60.57 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 AAAGGAGCTAATCGCCTTGC Chr17:71200488..71200508 60.84 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 GAAACAAAGGAGCCGTGACT Chr17:71200493..71200513 59.33 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000047407