Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI10908
Trapped Gene
Mycn (ENSMUSG00000037169)
Vector Insertion
Chr 12: 12947293 - 12948441
Public Clones P147D06 (ggtc) P147D06 (ggtc) P147D06 (ggtc)
Private Clones not available
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000656006 (Chr12:12948442..12948642 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000656006 (Chr12:12948442..12948642 -)
Downstram Exon
ENSMUSE00000229901 (Chr12:12946416..12947292 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon TGACCGGATTAGGAGTGAGG Chr12:12946844..12946863 60.06 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000656006 Chr12:12948442..12948642 No primer for this exon

*** Putative Vector Insertion (Chr 12: 12947293 - 12948441) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000229901 Chr12:12946416..12947292 TGACCGGATTAGGAGTGAGG Chr12:12946844..12946863 60.06 55
downstream ENSMUSE00000397736 Chr12:12942905..12944416 CTAATACTGGCCGCAAGAGC Chr12:12943321..12943340 60 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CAGTCTGCGGGAGGTAAGAA Chr12:12948433..12948453 60.39 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CAGTCTGCGGGAGGTAAGAA Chr12:12948433..12948453 60.39 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 TAATATCCCCCGAGCTTCAA Chr12:12948650..12948670 59.5 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 TAATATCCCCCGAGCTTCAA Chr12:12948650..12948670 59.5 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000037169