Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI11078
Trapped Gene
C1qbp (ENSMUSG00000018446)
Vector Insertion
Chr 11: 70792287 - 70793511
Public Clones G060B04 (ggtc) IST12661H11HMF1 (tigm) IST11402C8 (tigm) IST14903H2 (tigm)
IST12658D7 (tigm) IST14556C9 (tigm)
Private Clones OST263350 (lexicon) OST36670 (lexicon)
Severity of mutation (?) Insertion after 57% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000109520 (Chr11:70793512..70793605 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000109520 (Chr11:70793512..70793605 -)
Downstram Exon
ENSMUSE00000109524 (Chr11:70792191..70792286 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000485352 Chr11:70796227..70796467 No primer for this exon
upstream ENSMUSE00000318037 Chr11:70795672..70795822 No primer for this exon
upstream ENSMUSE00000109520 Chr11:70793512..70793605 No primer for this exon

*** Putative Vector Insertion (Chr 11: 70792287 - 70793511) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000109524 Chr11:70792191..70792286 No primer for this exon
downstream ENSMUSE00000109522 Chr11:70791930..70792052 No primer for this exon
downstream ENSMUSE00000318026 Chr11:70791360..70791746 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GCCAGTCTTTCTGTCCGAGA Chr11:70793467..70793487 60.53 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GAGAAGGAGGACGTGACTGG Chr11:70793451..70793471 59.83 60 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 AACAACAGCATCCCTCCAAC Chr11:70793562..70793582 59.97 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 TCCCTCCAACATTTGATCGT Chr11:70793552..70793572 60.32 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000018446