Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI11108
Trapped Gene
Cd97 (ENSMUSG00000002885)
Vector Insertion
Chr 8: 86253034 - 86253155
Public Clones G052D04 (ggtc)
Private Clones not available
Severity of mutation (?) Insertion after 26% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000212233 (Chr8:86253156..86253202 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000212233 (Chr8:86253156..86253202 -)
Downstram Exon
ENSMUSE00000212243 (Chr8:86252956..86253033 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000581482 Chr8:86265006..86265068 No primer for this exon
upstream ENSMUSE00000212241 Chr8:86258195..86258251 No primer for this exon
upstream ENSMUSE00000212250 Chr8:86257908..86258024 No primer for this exon
upstream ENSMUSE00000212248 Chr8:86257313..86257465 No primer for this exon
upstream ENSMUSE00000212237 Chr8:86255802..86255936 No primer for this exon
upstream ENSMUSE00000212245 Chr8:86254010..86254156 No primer for this exon
upstream ENSMUSE00000212240 Chr8:86253281..86253424 No primer for this exon
upstream ENSMUSE00000212233 Chr8:86253156..86253202 No primer for this exon

*** Putative Vector Insertion (Chr 8: 86253034 - 86253155) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000212243 Chr8:86252956..86253033 No primer for this exon
downstream ENSMUSE00000212251 Chr8:86252180..86252339 No primer for this exon
downstream ENSMUSE00000212236 Chr8:86251994..86252110 No primer for this exon
downstream ENSMUSE00000212235 Chr8:86251644..86251867 No primer for this exon
downstream ENSMUSE00000212246 Chr8:86249764..86249928 No primer for this exon
downstream ENSMUSE00000212249 Chr8:86249054..86249245 No primer for this exon
downstream ENSMUSE00000212234 Chr8:86248668..86248897 No primer for this exon
downstream ENSMUSE00000212239 Chr8:86248517..86248586 No primer for this exon
downstream ENSMUSE00000212247 Chr8:86248337..86248428 No primer for this exon
downstream ENSMUSE00000212244 Chr8:86248091..86248259 No primer for this exon
downstream ENSMUSE00000212242 Chr8:86247878..86247976 No primer for this exon
downstream ENSMUSE00000681668 Chr8:86247271..86247801 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CCTGTCTAATCGCCTTGCAG Chr8:86253090..86253110 60.92 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CCGTTCCCTACTTGGACACT Chr8:86253176..86253196 59.06 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000002885