Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI11281
Trapped Gene
Cs (ENSMUSG00000005683)
Vector Insertion
Chr 10: 127796356 - 127796489
Public Clones G075A01 (ggtc)
Private Clones not available
Severity of mutation (?) Insertion after 66% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000150362 (Chr10:127796226..127796355 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000150362 (Chr10:127796226..127796355 +)
Downstram Exon
ENSMUSE00000270596 (Chr10:127796490..127796591 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000368151 Chr10:127774790..127775015 No primer for this exon
upstream ENSMUSE00000150364 Chr10:127786809..127786859 No primer for this exon
upstream ENSMUSE00000150363 Chr10:127787489..127787596 No primer for this exon
upstream ENSMUSE00000150366 Chr10:127788255..127788320 No primer for this exon
upstream ENSMUSE00000150369 Chr10:127789730..127789861 No primer for this exon
upstream ENSMUSE00000150371 Chr10:127790079..127790267 No primer for this exon
upstream ENSMUSE00000150357 Chr10:127795390..127795589 No primer for this exon
upstream ENSMUSE00000150362 Chr10:127796226..127796355 No primer for this exon

*** Putative Vector Insertion (Chr 10: 127796356 - 127796489) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000270596 Chr10:127796490..127796591 No primer for this exon
downstream ENSMUSE00000150368 Chr10:127797000..127797209 No primer for this exon
downstream ENSMUSE00000350412 Chr10:127798024..127799534 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GGGCCTCTACATGGACTAGC Chr10:127796330..127796350 58.77 60 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GGGCCTCTACATGGACTAGC Chr10:127796330..127796350 58.77 60 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000005683