Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI11319
Trapped Gene
Trap1 (ENSMUSG00000005981)
Vector Insertion
Chr 16: 4049499 - 4052864
Public Clones G066A07 (ggtc)
Private Clones not available
Severity of mutation (?) Insertion after 59% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000127862 (Chr16:4052865..4052934 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000127862 (Chr16:4052865..4052934 -)
Downstram Exon
ENSMUSE00000127873 (Chr16:4049351..4049498 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000295399 Chr16:4077693..4077796 No primer for this exon
upstream ENSMUSE00000295391 Chr16:4068857..4069021 No primer for this exon
upstream ENSMUSE00000295385 Chr16:4068256..4068338 No primer for this exon
upstream ENSMUSE00000127861 Chr16:4065233..4065373 No primer for this exon
upstream ENSMUSE00000127865 Chr16:4062330..4062401 No primer for this exon
upstream ENSMUSE00000127855 Chr16:4060757..4060917 No primer for this exon
upstream ENSMUSE00000127874 Chr16:4056330..4056439 No primer for this exon
upstream ENSMUSE00000127854 Chr16:4055797..4055870 No primer for this exon
upstream ENSMUSE00000127868 Chr16:4054754..4054909 No primer for this exon
upstream ENSMUSE00000127856 Chr16:4053481..4053601 No primer for this exon
upstream ENSMUSE00000127862 Chr16:4052865..4052934 No primer for this exon

*** Putative Vector Insertion (Chr 16: 4049499 - 4052864) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000127873 Chr16:4049351..4049498 No primer for this exon
downstream ENSMUSE00000127872 Chr16:4045880..4046065 No primer for this exon
downstream ENSMUSE00000127864 Chr16:4045443..4045581 No primer for this exon
downstream ENSMUSE00000127866 Chr16:4044613..4044698 No primer for this exon
downstream ENSMUSE00000295289 Chr16:4043953..4044098 No primer for this exon
downstream ENSMUSE00000295279 Chr16:4040359..4040431 No primer for this exon
downstream ENSMUSE00000295269 Chr16:4039981..4040224 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CCTGAACCTCAGCAGAGAGC Chr16:4052888..4052908 60.28 60 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CCTGAACCTCAGCAGAGAGC Chr16:4052888..4052908 60.28 60 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000005981