Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI11583
Trapped Gene
Zcchc10 (ENSMUSG00000018239)
Vector Insertion
Chr 11: 53138244 - 53140770
Public Clones D143D05 (ggtc) D099H08 (ggtc) D130F01 (ggtc) M094C02 (ggtc) D130C01 (ggtc)
(ggtc) CMHD-GT_481H8-3 (cmhd) CMHD-GT_525A1-3 (cmhd) CMHD-GT_481F11-3 (cmhd)
CMHD-GT_481B2-3 (cmhd) CMHD-GT_421A12-3 (cmhd) CMHD-GT_481H10-3 (cmhd) (cmhd)
CMHD-GT_481C1-3 (cmhd) CMHD-GT_481D9-3 (cmhd) CMHD-GT_469B5-3 (cmhd) CMHD-GT_481E9-3 (cmhd)
CMHD-GT_472C12-3 (cmhd) CMHD-GT_481A11-3 (cmhd) IST14884A11 (tigm) IST14732C7 (tigm)
IST11273G3 (tigm) IST14106C1 (tigm) IST10559E4BBF1 (tigm) IST10559E4 (tigm)
IST14732C7 (tigm) IST14426E3 (tigm) IST14426E3 (tigm)
Private Clones OST471224 (lexicon) OST441836 (lexicon) OST423775 (lexicon) OST415951 (lexicon)
OST376933 (lexicon) OST374324 (lexicon) OST352035 (lexicon) OST346025 (lexicon)
OST310525 (lexicon) OST302640 (lexicon) OST296711 (lexicon) OST293430 (lexicon)
OST288063 (lexicon) OST281960 (lexicon) OST272940 (lexicon) OST270979 (lexicon)
OST264112 (lexicon) OST251771 (lexicon) OST242806 (lexicon) OST224088 (lexicon)
OST214774 (lexicon) OST184256 (lexicon) OST177630 (lexicon) OST172172 (lexicon)
OST171398 (lexicon) OST168459 (lexicon) OST158328 (lexicon) OST144224 (lexicon)
OST144192 (lexicon) OST142257 (lexicon) OST140726 (lexicon) OST133660 (lexicon)
OST129915 (lexicon) OST108590 (lexicon) OST100945 (lexicon) OST100476 (lexicon)
OST90893 (lexicon) OST89869 (lexicon) OST87435 (lexicon) OST82429 (lexicon)
OST79074 (lexicon) OST78874 (lexicon) OST77781 (lexicon) OST77710 (lexicon)
OST77266 (lexicon) OST73936 (lexicon) OST62962 (lexicon) OST54217 (lexicon)
OST48132 (lexicon) OST32760 (lexicon) OST30091 (lexicon)
Severity of mutation (?) Insertion after 8% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000103821 (Chr11:53138184..53138243 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000103821 (Chr11:53138184..53138243 +)
Downstram Exon
ENSMUSE00000103815 (Chr11:53140771..53140929 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000103821 Chr11:53138184..53138243 No primer for this exon

*** Putative Vector Insertion (Chr 11: 53138244 - 53140770) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000103815 Chr11:53140771..53140929 No primer for this exon
downstream ENSMUSE00000103823 Chr11:53144189..53144233 No primer for this exon
downstream ENSMUSE00000374181 Chr11:53145804..53146790 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GGCTCATAGCTCGAAGGAAA Chr11:53138223..53138243 59.55 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GGCTCATAGCTCGAAGGAAA Chr11:53138223..53138243 59.55 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000018239