Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI11591
Trapped Gene
Aebp2 (ENSMUSG00000030232)
Vector Insertion
Chr 6: 140596560 - 140596560
Public Clones P062A08 (ggtc)
Private Clones not available
Severity of mutation (?) Insertion after 82% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000430513 (Chr6:140596493..140596559 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CTGCCAGATGTATGGGTGAA Chr6:140596494..140596513 59.52 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000430513 (Chr6:140596493..140596559 +)
Downstram Exon
ENSMUSE00000430510 (Chr6:140596561..140596606 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CTGCCAGATGTATGGGTGAA Chr6:140596494..140596513 59.52 50 AAATGTTTGGGTCCAACAGC Chr6:140596605..140596624 59.84 45

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000608583 Chr6:140571184..140571579 CAGCCTGCCTACAAGTGTCA Chr6:140571449..140571468 60.05 55
upstream ENSMUSE00000714143 Chr6:140572210..140572865 AGGAGGAGGATGACGAGGAG Chr6:140572334..140572353 60.75 60
upstream ENSMUSE00000716728 Chr6:140572210..140572865 AGGAGGAGGATGACGAGGAG Chr6:140572334..140572353 60.75 60
upstream ENSMUSE00000706715 Chr6:140572819..140572865 No primer for this exon
upstream ENSMUSE00000608579 Chr6:140582210..140582417 CATTCGCTCCATACATGTCG Chr6:140582381..140582400 60.1 50
upstream ENSMUSE00000608582 Chr6:140582210..140582417 CATTCGCTCCATACATGTCG Chr6:140582381..140582400 60.1 50
upstream ENSMUSE00000196633 Chr6:140586181..140586288 GTGTTCGTTTGCTTGTGGAA Chr6:140586181..140586200 59.74 45
upstream ENSMUSE00000196631 Chr6:140590693..140590879 GAATGAACAAGCGGAGGAAA Chr6:140590832..140590851 60.19 45
upstream ENSMUSE00000196630 Chr6:140592469..140592593 AGACATCGAGCCATCTGCTT Chr6:140592513..140592532 59.98 50
upstream ENSMUSE00000196629 Chr6:140595331..140595398 GCTTTTGCTTCATTGGATGC Chr6:140595369..140595388 60.73 45
upstream ENSMUSE00000196632 Chr6:140596493..140596606 CTGCCAGATGTATGGGTGAA Chr6:140596494..140596513 59.52 50
upstream ENSMUSE00000430513 Chr6:140596493..140596559 CTGCCAGATGTATGGGTGAA Chr6:140596494..140596513 59.52 50

*** Putative Vector Insertion (Chr 6: 140596560 - 140596560) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000430510 Chr6:140596561..140596606 AAATGTTTGGGTCCAACAGC Chr6:140596605..140596624 59.84 45
downstream ENSMUSE00000430516 Chr6:140599277..140604002 GGTACCGCTCCCAACAGTAA Chr6:140600710..140600729 59.99 55
downstream ENSMUSE00000546755 Chr6:140599277..140599299 No primer for this exon
downstream ENSMUSE00000608581 Chr6:140599277..140599303 No primer for this exon
downstream ENSMUSE00000689236 Chr6:140625912..140625939 TCACCTTGGGAAATTGAGGA Chr6:140625942..140625961 60.43 45

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TTATCAACGTGACTGGGAAAA Chr6:140596555..140596576 58.13 38.1 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000030232