Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI11664
Trapped Gene
Rpl26 (ENSMUSG00000060938)
Vector Insertion
Chr 11: 68715111 - 68715127
Public Clones P093G08 (ggtc)
Private Clones not available
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000651955 (Chr11:68715097..68715126 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000651955 (Chr11:68715097..68715126 +)
Downstram Exon
ENSMUSE00000651953 (Chr11:68715112..68715314 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon CTGGTCCCCTTAACCCTCTC Chr11:68715317..68715336 59.93 60

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000651955 Chr11:68715097..68715126 No primer for this exon

*** Putative Vector Insertion (Chr 11: 68715111 - 68715127) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000651953 Chr11:68715112..68715314 CTGGTCCCCTTAACCCTCTC Chr11:68715317..68715336 59.93 60
downstream ENSMUSE00000463615 Chr11:68715772..68715944 TGATCTTCCTCCGAATGTGA Chr11:68715867..68715886 59.17 45
downstream ENSMUSE00000528761 Chr11:68715772..68715938 TGATCTTCCTCCGAATGTGA Chr11:68715867..68715886 59.17 45
downstream ENSMUSE00000472310 Chr11:68716681..68716821 TTCTCTCGCTGGACTCGTTC Chr11:68716781..68716800 60.68 55
downstream ENSMUSE00000508921 Chr11:68717867..68718035 CCGTGCACGAGATGTCTCTA Chr11:68718015..68718034 60.01 55
downstream ENSMUSE00000651954 Chr11:68717867..68718012 CCGTGCACGAGATGTCTCTA Chr11:68718015..68718034 60.01 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CGGAAGTCGCGTGTAGTTCT Chr11:68715074..68715094 60.45 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CGGAAGTCGCGTGTAGTTCT Chr11:68715074..68715094 60.45 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000060938