Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI11713
Trapped Gene
Mycbp2 (ENSMUSG00000033004)
Vector Insertion
Chr 14: 103516438 - 103517257
Public Clones M119F05 (ggtc)
Private Clones not available
Severity of mutation (?) Insertion after 98% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000253586 (Chr14:103517258..103517365 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
TGATTACGACCCCAGAGAGC Chr14:103517296..103517315 60.22 55 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000253586 (Chr14:103517258..103517365 -)
Downstram Exon
ENSMUSE00000253580 (Chr14:103516272..103516437 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
TGATTACGACCCCAGAGAGC Chr14:103517296..103517315 60.22 55 AGAAGACAGCCACCGAACAG Chr14:103516346..103516365 60.44 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000417209 Chr14:103745433..103746017 CGCTACCGGAGGATTTACAC Chr14:103745490..103745509 59.59 55
upstream ENSMUSE00000417204 Chr14:103722887..103722962 TTGAAGCGAAAACAGAAAAGC Chr14:103722914..103722934 59.64 38.1
upstream ENSMUSE00000417198 Chr14:103713516..103713731 CAGCTGCATCGAAAAACTCA Chr14:103713585..103713604 60.13 45
upstream ENSMUSE00000417193 Chr14:103705407..103705560 CTGCTGAATGTGCTTCAAGG Chr14:103705451..103705470 59.59 50
upstream ENSMUSE00000417188 Chr14:103697884..103698080 CAGTCACGCTTGCCAAACTA Chr14:103697889..103697908 60.05 50
upstream ENSMUSE00000417185 Chr14:103696397..103696639 AGGCAGTGTTGGTTGGAAAG Chr14:103696583..103696602 60.15 50
upstream ENSMUSE00000417181 Chr14:103696110..103696181 No primer for this exon
upstream ENSMUSE00000417177 Chr14:103694377..103694473 GCCCTGAGACACTGGAACAG Chr14:103694399..103694418 60.86 60
upstream ENSMUSE00000417172 Chr14:103690506..103690579 ATTGCCACACTGAAGGTCAA Chr14:103690560..103690579 59.14 45
upstream ENSMUSE00000417169 Chr14:103690235..103690373 TTACACGCCTGTGGCATATC Chr14:103690270..103690289 59.57 50
upstream ENSMUSE00000417162 Chr14:103688761..103688837 ATACTCGGTGCAGGACGAGA Chr14:103688792..103688811 60.82 55
upstream ENSMUSE00000417159 Chr14:103687760..103687964 ACAAGGTGGTCCTTCAGCAG Chr14:103687907..103687926 60.3 55
upstream ENSMUSE00000417156 Chr14:103686423..103686587 CGGCAGTCAAAGCCCTATAA Chr14:103686557..103686576 60.22 50
upstream ENSMUSE00000417154 Chr14:103684467..103684625 CAATGTGGACGAGACACTGG Chr14:103684490..103684509 60.15 55
upstream ENSMUSE00000417147 Chr14:103681815..103682019 GTGTGTGGAGACTGCACTGG Chr14:103681866..103681885 60.37 60
upstream ENSMUSE00000417143 Chr14:103675590..103675735 GAGTTGGACGGTCAAGAAGC Chr14:103675649..103675668 59.85 55
upstream ENSMUSE00000417139 Chr14:103675006..103675107 ACCGGGGGTTAATATTGAGG Chr14:103675078..103675097 59.9 50
upstream ENSMUSE00000417134 Chr14:103668575..103668682 AGCATAAACGGGACAAGCAC Chr14:103668579..103668598 60.14 50
upstream ENSMUSE00000417129 Chr14:103661919..103662038 AAGGACGCAAGCAAAATCAC Chr14:103661996..103662015 60.26 45
upstream ENSMUSE00000417127 Chr14:103659413..103659494 GACATGGGGATGTCAATTCC Chr14:103659415..103659434 59.99 50
upstream ENSMUSE00000417124 Chr14:103651626..103651743 TGGATGGACAGGTCTTCACA Chr14:103651641..103651660 60.09 50
upstream ENSMUSE00000417120 Chr14:103647579..103647783 TCCCATGCCTAACATTGGAT Chr14:103647708..103647727 60.15 45
upstream ENSMUSE00000417114 Chr14:103646964..103647117 AGACCTGCAAGGATTTGGTG Chr14:103646998..103647017 60.11 50
upstream ENSMUSE00000416437 Chr14:103642261..103642433 GTGTTACAATGCCGTGGTTG Chr14:103642387..103642406 59.89 50
upstream ENSMUSE00000416428 Chr14:103641539..103641664 TGGGCATTGCAAGTACAGAG Chr14:103641606..103641625 59.86 50
upstream ENSMUSE00000416421 Chr14:103641329..103641456 GGCTACTCTGCCCATTCAGT Chr14:103641420..103641439 59.31 55
upstream ENSMUSE00000416416 Chr14:103631743..103631834 TGAAACGGATGGAGACCTTC Chr14:103631783..103631802 60.05 50
upstream ENSMUSE00000416409 Chr14:103628572..103628706 GGCCAAGCGTCTATTACCAC Chr14:103628581..103628600 59.6 55
upstream ENSMUSE00000416403 Chr14:103626688..103626771 ACCGATGTAAATGCTGGTCA Chr14:103626709..103626728 59 45
upstream ENSMUSE00000416398 Chr14:103624636..103624732 ACCAGTGACGGCAGTACCTC Chr14:103624706..103624725 60.18 60
upstream ENSMUSE00000416393 Chr14:103623484..103623676 TTACTGCGCGTCTACACCTG Chr14:103623504..103623523 60.07 55
upstream ENSMUSE00000416388 Chr14:103622127..103622401 AGGAGTGCCACAACACCTTC Chr14:103622208..103622227 60.16 55
upstream ENSMUSE00000416382 Chr14:103619144..103619356 TGCTGCGATCGATTGTTAAG Chr14:103619222..103619241 59.98 45
upstream ENSMUSE00000416376 Chr14:103614537..103614728 GGAAGTGCTGGAGAAGATGC Chr14:103614674..103614693 59.96 55
upstream ENSMUSE00000416371 Chr14:103611740..103611946 GTCCCCTGATGCAATCTGTT Chr14:103611835..103611854 59.93 50
upstream ENSMUSE00000416366 Chr14:103610475..103610606 AGACGATTCACCCAGTGACA Chr14:103610513..103610532 59.1 50
upstream ENSMUSE00000416359 Chr14:103607966..103608144 TGTGCGCTTGAGGAACTATG Chr14:103608108..103608127 60.01 50
upstream ENSMUSE00000416352 Chr14:103605853..103605995 CACAGAGCTCTTGCTGTGGA Chr14:103605855..103605874 60.33 55
upstream ENSMUSE00000416347 Chr14:103604394..103604494 CCAGTCTGTTTTCCATGCTG Chr14:103604445..103604464 59.29 50
upstream ENSMUSE00000416341 Chr14:103603539..103603757 GCGTTCAATCCCAATCAGTC Chr14:103603660..103603679 60.47 50
upstream ENSMUSE00000416331 Chr14:103600429..103600654 AAGACGTCATCCGTTTGCTT Chr14:103600565..103600584 59.74 45
upstream ENSMUSE00000416323 Chr14:103599291..103599409 TGAATTTAGCCCTGGACCTG Chr14:103599296..103599315 60.07 50
upstream ENSMUSE00000416318 Chr14:103598650..103598737 CAGCCCTGATGAAGAAGGAT Chr14:103598666..103598685 59.24 50
upstream ENSMUSE00000416313 Chr14:103597857..103597906 ATTCTTGAAGAGGCTGCATTG Chr14:103597860..103597880 59.47 42.86
upstream ENSMUSE00000416307 Chr14:103596462..103596634 GAAACTTGCCGCTTCAAATC Chr14:103596536..103596555 59.83 45
upstream ENSMUSE00000416299 Chr14:103595455..103595599 TATGGCGATGTGGTTCATGT Chr14:103595468..103595487 59.81 45
upstream ENSMUSE00000416295 Chr14:103593801..103594010 AGCCAATGATCGTCAAGGAG Chr14:103593831..103593850 60.22 50
upstream ENSMUSE00000416291 Chr14:103590784..103590839 AAGAACTGCGTTTTGCATCA Chr14:103590798..103590817 59.47 40
upstream ENSMUSE00000416287 Chr14:103587716..103587831 GGCTATCGGGCTCTACACTG Chr14:103587746..103587765 59.86 60
upstream ENSMUSE00000416281 Chr14:103584801..103584898 TAGTCAAGCCGAAGGCAGAC Chr14:103584816..103584835 60.54 55
upstream ENSMUSE00000416275 Chr14:103584049..103584165 GAAAATTTGTGGCCAAGGAC Chr14:103584142..103584161 59.41 45
upstream ENSMUSE00000416269 Chr14:103581571..103581711 AAGCCTGGTGCCTCTCTTTT Chr14:103581610..103581629 60.38 50
upstream ENSMUSE00000416266 Chr14:103576473..103576547 ATGCTAGCCAAGGAGTCAGG Chr14:103576518..103576537 59.45 55
upstream ENSMUSE00000416260 Chr14:103573983..103574207 AACGAGATCACCCGTTCTTG Chr14:103574115..103574134 60.11 50
upstream ENSMUSE00000416255 Chr14:103573004..103573136 AGAGGCGGAAACCAGTACCT Chr14:103573018..103573037 60.13 55
upstream ENSMUSE00000416252 Chr14:103571784..103571906 CAGTGCCCAAGGATTTGACT Chr14:103571805..103571824 60.11 50
upstream ENSMUSE00000416249 Chr14:103569074..103569229 CTATTCAATCACGGCCCAAT Chr14:103569183..103569202 59.78 45
upstream ENSMUSE00000350096 Chr14:103554341..103555984 GAGGTCATCCTCCCCTAAGC Chr14:103555655..103555674 60.04 60
upstream ENSMUSE00000385459 Chr14:103553963..103554132 AGGATGGGCTGGTAACTGTG Chr14:103554058..103554077 59.99 55
upstream ENSMUSE00000399910 Chr14:103553143..103553387 AAGGCGTTTCTGAGGACCTT Chr14:103553184..103553203 60.25 50
upstream ENSMUSE00000336900 Chr14:103551337..103551504 CTCCTGATGCAAGTCCCAAT Chr14:103551381..103551400 60.07 50
upstream ENSMUSE00000253730 Chr14:103547725..103547882 CCCATAACCGACACTGACCT Chr14:103547850..103547869 59.84 55
upstream ENSMUSE00000253724 Chr14:103546050..103546246 GTCTTCCCTCTTGCGACATC Chr14:103546223..103546242 59.81 55
upstream ENSMUSE00000253717 Chr14:103545117..103545266 CTCCCGTAGAAGCAGAGGAA Chr14:103545162..103545181 59.57 55
upstream ENSMUSE00000253708 Chr14:103543471..103543631 ATAGTGATTGCAGGCGAAGC Chr14:103543573..103543592 60.38 50
upstream ENSMUSE00000253702 Chr14:103542360..103542516 CAGTCCGATCACCAGTTCCT Chr14:103542475..103542494 60.11 55
upstream ENSMUSE00000483356 Chr14:103541961..103542185 GTTTGACAGATGGCTCCACA Chr14:103542084..103542103 59.68 50
upstream ENSMUSE00000253689 Chr14:103540088..103540153 No primer for this exon
upstream ENSMUSE00000253679 Chr14:103538454..103538693 GAACTCCCAGGAGGGGATAA Chr14:103538635..103538654 60.26 55
upstream ENSMUSE00000467400 Chr14:103537937..103538035 ATCTCTGGAGACGCTGAACC Chr14:103538001..103538020 59.41 55
upstream ENSMUSE00000253664 Chr14:103535776..103535856 TGAAGGAACACATGGTTGGA Chr14:103535818..103535837 59.94 45
upstream ENSMUSE00000253657 Chr14:103534251..103534517 CCTCCTCTGACGCTTACTGC Chr14:103534383..103534402 60.16 60
upstream ENSMUSE00000465018 Chr14:103532999..103533282 CTGGCCAGTATCATCGGAGT Chr14:103533215..103533234 60.1 55
upstream ENSMUSE00000253645 Chr14:103530460..103530556 TACCTCCGACGAGGTGAGAG Chr14:103530536..103530555 60.4 60
upstream ENSMUSE00000253640 Chr14:103529148..103529267 ATTGCCTTGACCAAAATGGA Chr14:103529191..103529210 60.31 40
upstream ENSMUSE00000362574 Chr14:103527130..103527231 CTTGATCAGGACCATGTGGA Chr14:103527182..103527201 59.47 50
upstream ENSMUSE00000336423 Chr14:103526281..103526409 CATCGACGAACGAAGACTCA Chr14:103526291..103526310 59.98 50
upstream ENSMUSE00000385238 Chr14:103525839..103525971 TGGAATTCCGAGAACACACA Chr14:103525840..103525859 60.09 45
upstream ENSMUSE00000350046 Chr14:103523616..103523719 CACGAGTAGCTCGGAAGCAT Chr14:103523690..103523709 60.56 55
upstream ENSMUSE00000357280 Chr14:103522529..103522717 TGCTAAGATCGCCTGCAGTA Chr14:103522693..103522712 59.74 50
upstream ENSMUSE00000253600 Chr14:103521732..103521839 TGGAAAACAGATGGCTTGGT Chr14:103521774..103521793 60.49 45
upstream ENSMUSE00000253594 Chr14:103519723..103519932 CAGCTGGCTATGCCATGAAT Chr14:103519759..103519778 61.16 50
upstream ENSMUSE00000253586 Chr14:103517258..103517365 TGATTACGACCCCAGAGAGC Chr14:103517296..103517315 60.22 55

*** Putative Vector Insertion (Chr 14: 103516438 - 103517257) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000253580 Chr14:103516272..103516437 AGAAGACAGCCACCGAACAG Chr14:103516346..103516365 60.44 55
downstream ENSMUSE00000415863 Chr14:103512630..103513452 CACCCGAGAGCAAACTCTTC Chr14:103513346..103513365 59.99 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GGTAGGCAGACGCTAGACAGA Chr14:103517236..103517257 59.65 57.14 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000033004