Tools for the biologist enabling optimized use of gene trap clones
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About
UniTrap UNI11784
Trapped Gene
Zfp652 (
ENSMUSG00000075595
)
Vector Insertion
Chr 11: 95614337 - 95614675
Public Clones
A026E05 (ggtc)
Private Clones
not available
Severity of mutation
(?)
Insertion after 57% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000674206 (Chr11:95614189..95614336 +)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
CCATGGCTCATGTACGAAAA
Chr11:95614307..95614326
59.54
45
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000674206 (Chr11:95614189..95614336 +)
Downstram Exon
ENSMUSE00000674205 (Chr11:95614676..95614791 +)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
CCATGGCTCATGTACGAAAA
Chr11:95614307..95614326
59.54
45
GGAGTGCACCTTGAGTGACA
Chr11:95614758..95614777
59.87
55
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000674202
Chr11:95573987..95574047
No primer for this exon
upstream
ENSMUSE00000674201
Chr11:95610314..95611461
CCGTAGGAAAAGTGCAGAGC
Chr11:95611197..95611216
60.01
55
upstream
ENSMUSE00000268212
Chr11:95610381..95611461
CCGTAGGAAAAGTGCAGAGC
Chr11:95611197..95611216
60.01
55
upstream
ENSMUSE00000674206
Chr11:95614189..95614336
CCATGGCTCATGTACGAAAA
Chr11:95614307..95614326
59.54
45
*** Putative Vector Insertion (Chr 11: 95614337 - 95614675) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000674205
Chr11:95614676..95614791
GGAGTGCACCTTGAGTGACA
Chr11:95614758..95614777
59.87
55
downstream
ENSMUSE00000674204
Chr11:95615225..95615369
CACTGGCACATGAACTGCTT
Chr11:95615313..95615332
59.9
50
downstream
ENSMUSE00000674200
Chr11:95624853..95633667
CTCCGCAGTGGAGTTCTTTC
Chr11:95629170..95629189
59.99
55
downstream
ENSMUSE00000674203
Chr11:95624853..95626025
CACCAAAAGCTGAACCGAAT
Chr11:95625787..95625806
60.11
45
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
TGCTCTCTTAATCGCCTTGC
Chr11:95614380..95614400
60.63
50
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
TTCTCCACCTGCAGACTCCT
Chr11:95614356..95614376
59.99
55
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000075595