Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI11884
Trapped Gene
Itgb7 (ENSMUSG00000001281)
Vector Insertion
Chr 15: 102058033 - 102062281
Public Clones M042E06 (ggtc) D113A12 (ggtc) D113A12 (ggtc) (egtc) IST10239B5 (tigm)
IST10239B5 (tigm) IST11338E6 (tigm) IST10918B1 (tigm) IST14904B4 (tigm)
IST13841A7 (tigm) IST14725E1 (tigm) IST13199F8 (tigm) IST10282D6 (tigm)
Private Clones OST419170 (lexicon) OST335962 (lexicon) OST209897 (lexicon) OST207965 (lexicon)
OST182400 (lexicon) OST52047 (lexicon) OST33999 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000284907 (Chr15:102062282..102062339 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000284907 (Chr15:102062282..102062339 -)
Downstram Exon
ENSMUSE00000284896 (Chr15:102057829..102058032 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000284907 Chr15:102062282..102062339 No primer for this exon

*** Putative Vector Insertion (Chr 15: 102058033 - 102062281) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000284896 Chr15:102057829..102058032 No primer for this exon
downstream ENSMUSE00000133153 Chr15:102054989..102055190 No primer for this exon
downstream ENSMUSE00000133162 Chr15:102054733..102054903 No primer for this exon
downstream ENSMUSE00000133165 Chr15:102053762..102054003 No primer for this exon
downstream ENSMUSE00000133159 Chr15:102053072..102053230 No primer for this exon
downstream ENSMUSE00000133157 Chr15:102052773..102052868 No primer for this exon
downstream ENSMUSE00000133155 Chr15:102052520..102052609 No primer for this exon
downstream ENSMUSE00000133160 Chr15:102049605..102049751 No primer for this exon
downstream ENSMUSE00000284830 Chr15:102048928..102049121 No primer for this exon
downstream ENSMUSE00000284820 Chr15:102048205..102048428 No primer for this exon
downstream ENSMUSE00000284808 Chr15:102047678..102047897 No primer for this exon
downstream ENSMUSE00000133158 Chr15:102047323..102047528 No primer for this exon
downstream ENSMUSE00000133161 Chr15:102046903..102047063 No primer for this exon
downstream ENSMUSE00000381055 Chr15:102046429..102046698 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CTCCTCTCACCCAGGCTAATC Chr15:102059225..102059246 60.22 57.14 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CTCCTCAAGCACCTGTGAGT Chr15:102062274..102062294 58.01 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 TGTCACTTCCTCCTCTGCTG Chr15:102059349..102059369 59.12 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 CTGCTGCCTCTCCATCTGTA Chr15:102062335..102062355 59.14 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000001281