Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI12032
Trapped Gene
Arid3b (ENSMUSG00000004661)
Vector Insertion
Chr 9: 57643913 - 57644160
Public Clones A062C06 (ggtc)
Private Clones not available
Severity of mutation (?) Insertion after 82% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000636508 (Chr9:57643914..57644159 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000636508 (Chr9:57643914..57644159 -)
Downstram Exon
ENSMUSE00000467845 (Chr9:57643914..57644159 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000698368 Chr9:57684489..57684600 No primer for this exon
upstream ENSMUSE00000444631 Chr9:57681423..57682043 No primer for this exon
upstream ENSMUSE00000478601 Chr9:57681423..57682039 No primer for this exon
upstream ENSMUSE00000464653 Chr9:57658216..57658287 No primer for this exon
upstream ENSMUSE00000218667 Chr9:57657972..57658044 No primer for this exon
upstream ENSMUSE00000534120 Chr9:57657968..57658044 No primer for this exon
upstream ENSMUSE00000636512 Chr9:57653238..57653346 No primer for this exon
upstream ENSMUSE00000486564 Chr9:57645849..57646032 No primer for this exon
upstream ENSMUSE00000636511 Chr9:57645849..57646032 No primer for this exon
upstream ENSMUSE00000484441 Chr9:57644279..57644595 No primer for this exon
upstream ENSMUSE00000636510 Chr9:57644279..57644595 No primer for this exon
upstream ENSMUSE00000467845 Chr9:57643914..57644159 No primer for this exon
upstream ENSMUSE00000636508 Chr9:57643914..57644159 No primer for this exon

*** Putative Vector Insertion (Chr 9: 57643913 - 57644160) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000465630 Chr9:57642743..57642841 No primer for this exon
downstream ENSMUSE00000636507 Chr9:57642494..57642841 No primer for this exon
downstream ENSMUSE00000698353 Chr9:57640323..57640492 No primer for this exon
downstream ENSMUSE00000464736 Chr9:57638320..57640492 No primer for this exon
downstream ENSMUSE00000476350 Chr9:57638269..57638422 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CAGTTCCTTTGCAGGTGATG Chr9:57644152..57644172 59.29 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CAGTTCCTTTGCAGGTGATG Chr9:57644152..57644172 59.29 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000004661