Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI12091
Trapped Gene
Dppa2 (ENSMUSG00000072419)
Vector Insertion
Chr 16: 48317597 - 48318745
Public Clones W202C01 (ggtc)
Private Clones not available
Severity of mutation (?) Insertion after 91% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000699684 (Chr16:48317401..48317596 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CGGCCTGTATTATCCCAGAA Chr16:48317533..48317552 59.92 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000699684 (Chr16:48317401..48317596 +)
Downstram Exon
ENSMUSE00000699682 (Chr16:48318746..48319625 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CGGCCTGTATTATCCCAGAA Chr16:48317533..48317552 59.92 50 GTTAAAATGCAACGGGCTGT Chr16:48319027..48319046 60 45

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000699690 Chr16:48310590..48310652 TCATACTTCGGCCTGGAGAC Chr16:48310623..48310642 60.22 55
upstream ENSMUSE00000629029 Chr16:48311688..48311823 TTTAACGCTGGTCCCCTTTA Chr16:48311726..48311745 59.58 45
upstream ENSMUSE00000699688 Chr16:48313996..48314147 TGCCTCCTATTAGGGACGTG Chr16:48314071..48314090 60.09 55
upstream ENSMUSE00000699687 Chr16:48314242..48314295 No primer for this exon
upstream ENSMUSE00000699686 Chr16:48315775..48316030 AGGAGAGTGGTGTCCTGGAA Chr16:48315881..48315900 59.68 55
upstream ENSMUSE00000699684 Chr16:48317401..48317596 CGGCCTGTATTATCCCAGAA Chr16:48317533..48317552 59.92 50

*** Putative Vector Insertion (Chr 16: 48317597 - 48318745) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000699682 Chr16:48318746..48319625 GTTAAAATGCAACGGGCTGT Chr16:48319027..48319046 60 45

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CTCTCTTGGCATGGGAAACT Chr16:48317613..48317633 59.28 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CTCTCTTGGCATGGGAAACT Chr16:48317613..48317633 59.28 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000072419