Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI12301
Trapped Gene
Cep152 (ENSMUSG00000068394)
Vector Insertion
Chr 2: 125440168 - 125444108
Public Clones F15228 (ggtc)
Private Clones not available
Severity of mutation (?) Insertion after 13% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000499789 (Chr2:125444109..125444277 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
GGCTTGCAGCAACAGTTTTT Chr2:125444123..125444142 60.43 45 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000499789 (Chr2:125444109..125444277 -)
Downstram Exon
ENSMUSE00000504369 (Chr2:125440024..125440167 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
GGCTTGCAGCAACAGTTTTT Chr2:125444123..125444142 60.43 45 GTTCGCTGTCCTTCAGCTTT Chr2:125440044..125440063 59.62 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000510503 Chr2:125450612..125450849 GCGTTCGAGTCTCCTACACC Chr2:125450713..125450732 59.87 60
upstream ENSMUSE00000511447 Chr2:125446909..125447002 GTCATTGGAGTTTGGCAGTG Chr2:125446974..125446993 59.14 50
upstream ENSMUSE00000500963 Chr2:125446023..125446126 ACAGACCTCCCTCACGACAT Chr2:125446092..125446111 59.56 55
upstream ENSMUSE00000501730 Chr2:125445733..125445805 GAAGTGGATTGAGCGGGATA Chr2:125445759..125445778 60.04 50
upstream ENSMUSE00000498953 Chr2:125445402..125445626 AGCCAGCGTGATTACGTTTT Chr2:125445428..125445447 59.78 45
upstream ENSMUSE00000499789 Chr2:125444109..125444277 GGCTTGCAGCAACAGTTTTT Chr2:125444123..125444142 60.43 45

*** Putative Vector Insertion (Chr 2: 125440168 - 125444108) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000504369 Chr2:125440024..125440167 GTTCGCTGTCCTTCAGCTTT Chr2:125440044..125440063 59.62 50
downstream ENSMUSE00000505351 Chr2:125438673..125438812 TTCAAGCTGCATCTCCCTCT Chr2:125438708..125438727 60.1 50
downstream ENSMUSE00000503305 Chr2:125436744..125436944 CTCCACCAGTTGCTGTTTCA Chr2:125436860..125436879 59.87 50
downstream ENSMUSE00000504278 Chr2:125431072..125431219 AGTTGTTGCACGTGGTCCTT Chr2:125431157..125431176 60.61 50
downstream ENSMUSE00000493500 Chr2:125428578..125428669 ACTTAGGACGTGGGCCTTCT Chr2:125428580..125428599 60.13 55
downstream ENSMUSE00000494536 Chr2:125426885..125427048 GTCTCCTTCGGAGTCACCTG Chr2:125426940..125426959 59.83 60
downstream ENSMUSE00000491381 Chr2:125420609..125420807 AGATGTCGCTTCACGGAGTT Chr2:125420675..125420694 59.87 50
downstream ENSMUSE00000560013 Chr2:125415846..125415968 GAAGATTCGAGCCATAACTGC Chr2:125415894..125415914 58.94 47.62
downstream ENSMUSE00000560012 Chr2:125415192..125415301 GCTTCCTGCTTATCGTGGTC Chr2:125415179..125415198 59.84 55
downstream ENSMUSE00000560011 Chr2:125413629..125413757 ACTGGGTCCCCTCATAGACC Chr2:125413617..125413636 60.19 60
downstream ENSMUSE00000560010 Chr2:125413337..125413463 CCAGGTAACACTCCTGCACA Chr2:125413386..125413405 59.74 55
downstream ENSMUSE00000560006 Chr2:125412067..125412210 TGCGCACGTATTCTTCTCTG Chr2:125412152..125412171 60.16 50
downstream ENSMUSE00000560003 Chr2:125409528..125409800 GACTGCCACTCCTTTTCCAG Chr2:125409735..125409754 59.84 55
downstream ENSMUSE00000560001 Chr2:125407432..125407563 TTCTGCACAGCTGTTTCCAC Chr2:125407522..125407541 60.03 50
downstream ENSMUSE00000560000 Chr2:125405110..125405760 CTGTGAGTCCCCACCGTACT Chr2:125405235..125405254 60.03 60
downstream ENSMUSE00000559998 Chr2:125402450..125402591 CTGAGCACAGCGTACAGAGG Chr2:125402462..125402481 59.79 60
downstream ENSMUSE00000559996 Chr2:125397405..125397569 AAGCAGTGTCCACAGCGTAA Chr2:125397502..125397521 59.51 50
downstream ENSMUSE00000559993 Chr2:125395291..125395387 ATTCTTCAACCACCCTGCTG Chr2:125395339..125395358 60.11 50
downstream ENSMUSE00000559990 Chr2:125394560..125394663 No primer for this exon
downstream ENSMUSE00000559989 Chr2:125392269..125392425 CATCTTCCGTGCAGTTTCCT Chr2:125392306..125392325 60.26 50
downstream ENSMUSE00000559988 Chr2:125391945..125392057 No primer for this exon
downstream ENSMUSE00000559987 Chr2:125388824..125390133 AGATGGGTGTCCCAGAGTTG Chr2:125389258..125389277 59.96 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 AGGCTTGCAGCAACAGTTTT Chr2:125444122..125444142 60.06 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AGGCTTGCAGCAACAGTTTT Chr2:125444122..125444142 60.06 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000068394