Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI12357
Trapped Gene
Cct3 (ENSMUSG00000001416)
Vector Insertion
Chr 3: 88103326 - 88104721
Public Clones M050A08 (ggtc) CMHD-GT_430A7-3 (cmhd) NAISTrap_26v1079 (NAISTrap)
Private Clones OST425370 (lexicon)
Severity of mutation (?) Insertion after 6% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000175674 (Chr3:88103264..88103325 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000175674 (Chr3:88103264..88103325 +)
Downstram Exon
ENSMUSE00000175685 (Chr3:88104722..88104772 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000360495 Chr3:88101061..88101188 No primer for this exon
upstream ENSMUSE00000175674 Chr3:88103264..88103325 No primer for this exon

*** Putative Vector Insertion (Chr 3: 88103326 - 88104721) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000175685 Chr3:88104722..88104772 No primer for this exon
downstream ENSMUSE00000175681 Chr3:88104851..88104913 No primer for this exon
downstream ENSMUSE00000175675 Chr3:88106690..88106786 No primer for this exon
downstream ENSMUSE00000175678 Chr3:88108935..88109052 No primer for this exon
downstream ENSMUSE00000175683 Chr3:88113083..88113269 No primer for this exon
downstream ENSMUSE00000175677 Chr3:88115595..88115744 No primer for this exon
downstream ENSMUSE00000175680 Chr3:88117164..88117296 No primer for this exon
downstream ENSMUSE00000292867 Chr3:88117478..88117559 No primer for this exon
downstream ENSMUSE00000175682 Chr3:88122268..88122448 No primer for this exon
downstream ENSMUSE00000175673 Chr3:88124382..88124627 No primer for this exon
downstream ENSMUSE00000175679 Chr3:88124936..88125067 No primer for this exon
downstream ENSMUSE00000175676 Chr3:88125366..88125688 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TCTCCTTAATCGCCTTGCAG Chr3:88103371..88103391 60.48 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TTCTGGCTGACTCTCCTCGT Chr3:88103360..88103380 60.13 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000001416