Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI12452
Trapped Gene
Mcm7 (ENSMUSG00000029730)
Vector Insertion
Chr 5: 138610953 - 138611544
Public Clones (sanger) (sanger) M001C02 (ggtc) PST23730-NR (escells) PST16765-NL (escells)
Private Clones OST241326 (lexicon)
Severity of mutation (?) Insertion after 5% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000304321 (Chr5:138611545..138611624 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000304321 (Chr5:138611545..138611624 -)
Downstram Exon
ENSMUSE00000192143 (Chr5:138610788..138610952 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon GTCGACCAACTCAGGGTCAT Chr5:138610850..138610869 59.97 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000686007 Chr5:138612954..138613090 GATGGCGCTTAAGGACTACG Chr5:138612966..138612985 59.87 55
upstream ENSMUSE00000304321 Chr5:138611545..138611624 No primer for this exon

*** Putative Vector Insertion (Chr 5: 138610953 - 138611544) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000192143 Chr5:138610788..138610952 GTCGACCAACTCAGGGTCAT Chr5:138610850..138610869 59.97 55
downstream ENSMUSE00000192142 Chr5:138610577..138610701 TCAGCCGGTGTTCAATGTAA Chr5:138610634..138610653 60.11 45
downstream ENSMUSE00000192155 Chr5:138610326..138610506 GGCCTCGGAAATACAACTCA Chr5:138610465..138610484 60.07 50
downstream ENSMUSE00000304275 Chr5:138610038..138610175 AACAAATTTGGAGCCACGAG Chr5:138610046..138610065 60.11 45
downstream ENSMUSE00000192141 Chr5:138609561..138609710 GCTGGGCAATTCTTGTGTTC Chr5:138609610..138609629 60.65 50
downstream ENSMUSE00000192152 Chr5:138609286..138609400 CAGTGGGCTTCCAGGTAAGT Chr5:138609344..138609363 59.21 55
downstream ENSMUSE00000192150 Chr5:138609069..138609200 AGGAGACTGGTCCACACCAC Chr5:138609066..138609085 60.01 60
downstream ENSMUSE00000192151 Chr5:138608779..138608862 CACACCAGGGTCTCCCATAA Chr5:138608803..138608822 60.77 55
downstream ENSMUSE00000192145 Chr5:138608274..138608667 ATTGAGCGTGGTGAGAATCC Chr5:138608410..138608429 60.08 50
downstream ENSMUSE00000192147 Chr5:138607911..138607994 GTGCTGGTGGACATAGGTGA Chr5:138607939..138607958 59.55 55
downstream ENSMUSE00000192148 Chr5:138607138..138607306 TCCGGGCAGAAGTATAGGTG Chr5:138607151..138607170 60.09 55
downstream ENSMUSE00000192153 Chr5:138606280..138606389 TCCTTCGACATCTCCATCAA Chr5:138606291..138606310 59.17 45
downstream ENSMUSE00000535291 Chr5:138605817..138606148 TGAAGCCACGAGATATGCAG Chr5:138606017..138606036 59.97 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GGGACCCAGTTGGTAAGTCTC Chr5:138611534..138611555 59.85 57.14 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AACTTGCGTGACTGGGAAAA Chr5:138611479..138611499 60.67 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 TGAGCTTGGTAATCGCCTTG Chr5:138611563..138611583 61.29 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 AGAATGAGCTTGGCGTGACT Chr5:138611567..138611587 60.02 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000029730