Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI12498
Trapped Gene
Tmem208 (ENSMUSG00000014856)
Vector Insertion
Chr 8: 107852593 - 107852671
Public Clones W174F02 (ggtc)
Private Clones not available
Severity of mutation (?) Insertion after 90% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000214305 (Chr8:107852508..107852592 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000214305 (Chr8:107852508..107852592 +)
Downstram Exon
ENSMUSE00000680031 (Chr8:107852672..107852802 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000233810 Chr8:107850277..107850374 No primer for this exon
upstream ENSMUSE00000680032 Chr8:107850362..107850374 No primer for this exon
upstream ENSMUSE00000214307 Chr8:107850951..107851046 No primer for this exon
upstream ENSMUSE00000214304 Chr8:107852070..107852129 No primer for this exon
upstream ENSMUSE00000214306 Chr8:107852222..107852358 No primer for this exon
upstream ENSMUSE00000214305 Chr8:107852508..107852592 No primer for this exon

*** Putative Vector Insertion (Chr 8: 107852593 - 107852671) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000402225 Chr8:107852672..107852951 No primer for this exon
downstream ENSMUSE00000680031 Chr8:107852672..107852802 No primer for this exon
downstream ENSMUSE00000634764 Chr8:107858543..107858774 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CACTGCTCACCAGTCTTTGC Chr8:107852622..107852642 59.62 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CACTGCTCACCAGTCTTTGC Chr8:107852622..107852642 59.62 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000014856