Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI12521
Trapped Gene
Rps24 (ENSMUSG00000025290)
Vector Insertion
Chr 14: 25312842 - 25314651
Public Clones W162C04 (ggtc) PST20297-NR (escells)
Private Clones OST58843 (lexicon)
Severity of mutation (?) Insertion after 100% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000691466 (Chr14:25312839..25312841 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000691466 (Chr14:25312839..25312841 +)
Downstram Exon
ENSMUSE00000691469 (Chr14:25314652..25314671 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000691481 Chr14:25309940..25309980 TCTTTTCCTCCTCTCCAGCTC Chr14:25309941..25309961 60.08 52.38
upstream ENSMUSE00000619464 Chr14:25310972..25311037 ACCAACCGTCTGCTTCAGAG Chr14:25311008..25311027 60.44 55
upstream ENSMUSE00000619463 Chr14:25311129..25311338 GGGAAGGCAACAGTACCAAA Chr14:25311150..25311169 59.97 50
upstream ENSMUSE00000619462 Chr14:25312605..25312715 AAAGACCTCCCGAAAACAGC Chr14:25312625..25312644 60.61 50
upstream ENSMUSE00000691466 Chr14:25312839..25312841 No primer for this exon

*** Putative Vector Insertion (Chr 14: 25312842 - 25314651) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000691469 Chr14:25314652..25314671 No primer for this exon
downstream ENSMUSE00000650494 Chr14:25314997..25315067 AAACATCAAGTCACCGCAGA Chr14:25315032..25315051 59.29 45
downstream ENSMUSE00000691468 Chr14:25314997..25315067 AAACATCAAGTCACCGCAGA Chr14:25315032..25315051 59.29 45

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GTAATCGCCTTGCAGCACAT Chr14:25312892..25312912 61.2 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TGGTGTTTTTCTTTCCCCTCT Chr14:25312807..25312828 59.96 42.86 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000025290