Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI12646
Trapped Gene
Map2k7 (ENSMUSG00000002948)
Vector Insertion
Chr 8: 4238864 - 4239034
Public Clones A027F04 (ggtc)
Private Clones not available
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000713349 (Chr8:4238829..4239033 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000713349 (Chr8:4238829..4239033 +)
Downstram Exon
ENSMUSE00000706648 (Chr8:4238865..4239033 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000362367 Chr8:4238761..4239033 No primer for this exon
upstream ENSMUSE00000387676 Chr8:4238829..4239033 No primer for this exon
upstream ENSMUSE00000713349 Chr8:4238829..4239033 No primer for this exon

*** Putative Vector Insertion (Chr 8: 4238864 - 4239034) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000706648 Chr8:4238865..4239033 No primer for this exon
downstream ENSMUSE00000686220 Chr8:4240105..4240289 No primer for this exon
downstream ENSMUSE00000210196 Chr8:4240688..4240735 No primer for this exon
downstream ENSMUSE00000686222 Chr8:4243264..4243445 No primer for this exon
downstream ENSMUSE00000467640 Chr8:4243304..4243445 No primer for this exon
downstream ENSMUSE00000507408 Chr8:4243304..4243445 No primer for this exon
downstream ENSMUSE00000210200 Chr8:4243553..4243619 No primer for this exon
downstream ENSMUSE00000210193 Chr8:4243743..4243856 No primer for this exon
downstream ENSMUSE00000210191 Chr8:4243951..4244070 No primer for this exon
downstream ENSMUSE00000210195 Chr8:4244229..4244336 No primer for this exon
downstream ENSMUSE00000210194 Chr8:4244427..4244606 No primer for this exon
downstream ENSMUSE00000210202 Chr8:4244695..4244775 No primer for this exon
downstream ENSMUSE00000210198 Chr8:4244861..4245003 No primer for this exon
downstream ENSMUSE00000210199 Chr8:4245543..4245588 No primer for this exon
downstream ENSMUSE00000461972 Chr8:4245695..4247897 No primer for this exon
downstream ENSMUSE00000486520 Chr8:4245695..4245825 No primer for this exon
downstream ENSMUSE00000686219 Chr8:4245695..4247896 No primer for this exon
downstream ENSMUSE00000686221 Chr8:4245695..4245841 No primer for this exon
downstream ENSMUSE00000686227 Chr8:4245695..4246107 No primer for this exon
downstream ENSMUSE00000686228 Chr8:4247657..4247897 No primer for this exon
downstream ENSMUSE00000686217 Chr8:4248214..4248352 No primer for this exon
downstream ENSMUSE00000423049 Chr8:4249205..4249341 No primer for this exon
downstream ENSMUSE00000686216 Chr8:4249205..4249341 No primer for this exon
downstream ENSMUSE00000423045 Chr8:4249426..4249746 No primer for this exon
downstream ENSMUSE00000686234 Chr8:4249426..4249592 No primer for this exon
downstream ENSMUSE00000504610 Chr8:4250267..4250381 No primer for this exon
downstream ENSMUSE00000505630 Chr8:4250467..4250625 No primer for this exon
downstream ENSMUSE00000686213 Chr8:4250849..4250921 No primer for this exon
downstream ENSMUSE00000686212 Chr8:4251293..4251423 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
  No PCR available

Come back to gene ENSMUSG00000002948