Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI12836
Trapped Gene
Glod5 (ENSMUSG00000031163)
Vector Insertion
Chr X: 7582985 - 7592343
Public Clones CMHD-GT_117A5-3 (cmhd) CMHD-GT_400H7-3 (cmhd) CMHD-GT_450E4-3 (cmhd) CMHD-GT_301A12-3 (cmhd)
CMHD-GT_391F8-3 (cmhd) CMHD-GT_121H12-3 (cmhd) CMHD-GT_403B2-3 (cmhd) CMHD-GT_374D11-3 (cmhd)
PSTVUpb6d1 (vanderbilt) IST14595E8 (tigm) IST14063E12 (tigm) IST11774E6 (tigm)
IST14551E2 (tigm) IST10927E7 (tigm) IST14272C2 (tigm) IST12883D9 (tigm)
IST13274F12 (tigm) IST13308E5 (tigm) IST13731H8 (tigm) IST14665A6 (tigm)
IST14049H8 (tigm) IST14544G12 (tigm) IST14520B5 (tigm) IST11324F5 (tigm)
IST14000D3 (tigm) IST14887C5 (tigm) IST14330B3 (tigm) IST13740A10 (tigm)
IST13541F2 (tigm) IST14940E1 (tigm) IST14667B7 (tigm) IST12315H1 (tigm)
IST10927E7 (tigm) IST13412G5 (tigm) IST12125F7 (tigm) IST10826A11HMF1 (tigm)
IST14887C5 (tigm) IST14667B7 (tigm) IST10826A11 (tigm)
Private Clones OST428938 (lexicon) OST371889 (lexicon) OST329207 (lexicon) OST309023 (lexicon)
OST307540 (lexicon) OST302477 (lexicon) OST296284 (lexicon) OST296257 (lexicon)
OST271582 (lexicon) OST270474 (lexicon) OST266738 (lexicon) OST265606 (lexicon)
OST253565 (lexicon) OST233145 (lexicon) OST217693 (lexicon) OST216040 (lexicon)
OST191446 (lexicon) OST180682 (lexicon) OST177753 (lexicon) OST177059 (lexicon)
OST177030 (lexicon) OST175389 (lexicon) OST173660 (lexicon) OST172562 (lexicon)
OST141876 (lexicon) OST141780 (lexicon) OST135989 (lexicon) OST130568 (lexicon)
OST123700 (lexicon) OST123597 (lexicon) OST101773 (lexicon) OST99567 (lexicon)
OST85791 (lexicon) OST85784 (lexicon) OST74758 (lexicon) OST57883 (lexicon)
OST38624 (lexicon) OST36420 (lexicon) OST31659 (lexicon) OST31462 (lexicon)
OST31452 (lexicon) OST29425 (lexicon) OST29377 (lexicon) OST28788 (lexicon)
OST28566 (lexicon) OST28170 (lexicon) OST28002 (lexicon) OST27900 (lexicon)
OST26864 (lexicon) OST24482 (lexicon) OST24339 (lexicon) OST22577 (lexicon)
OST20553 (lexicon) OST18075 (lexicon) OST16682 (lexicon) OST14038 (lexicon)
OST13889 (lexicon) OST12931 (lexicon) OST11869 (lexicon) OST10562 (lexicon)
OST9688 (lexicon) OST9559 (lexicon) OST2479 (lexicon)
Severity of mutation (?) Insertion after 37% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000206987 (ChrX:7592344..7592481 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CCTTCCCCGTGTCTTATCTG ChrX:7592438..7592457 59.54 55 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000206987 (ChrX:7592344..7592481 -)
Downstram Exon
ENSMUSE00000206984 (ChrX:7582829..7582984 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CCTTCCCCGTGTCTTATCTG ChrX:7592438..7592457 59.54 55 ACACAATGCTTTCCGGTTTC ChrX:7582942..7582961 59.98 45

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000206988 ChrX:7595543..7595618 TGCCTTCCAGTCTACCAGGA ChrX:7595573..7595592 60.79 55
upstream ENSMUSE00000206987 ChrX:7592344..7592481 CCTTCCCCGTGTCTTATCTG ChrX:7592438..7592457 59.54 55

*** Putative Vector Insertion (Chr X: 7582985 - 7592343) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000206984 ChrX:7582829..7582984 ACACAATGCTTTCCGGTTTC ChrX:7582942..7582961 59.98 45
downstream ENSMUSE00000240769 ChrX:7581327..7581628 TGTCCGGATCTCGGAAGTAG ChrX:7581519..7581538 60.21 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TAATCGCCTTGCAGCACATC ChrX:7592272..7592292 62.25 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GGCATGGAGGTCACTACTTTC ChrX:7592345..7592366 58.66 52.38 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 ACATAATCGCCTTGCAGCAC ChrX:7592414..7592434 61.2 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 TGTAGACCGTGACTGGGAAA ChrX:7592418..7592438 59.13 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000031163