Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI12880
Trapped Gene
Gabarapl2 (ENSMUSG00000031950)
Vector Insertion
Chr 8: 114466575 - 114476214
Public Clones D185B07 (ggtc) D096H09 (ggtc) D185B07 (ggtc) D095D08 (ggtc) D134C11 (ggtc)
CMHD-GT_162H5-3 (cmhd) CMHD-GT_393E12-3 (cmhd) CMHD-GT_451D4-3 (cmhd) CMHD-GT_382E2-3 (cmhd)
CMHD-GT_541C4-3 (cmhd) CMHD-GT_356C7-3 (cmhd) CMHD-GT_418F6-3 (cmhd) CMHD-GT_384A11-3 (cmhd)
CMHD-GT_491H2-3 (cmhd) PSTVUpb14e1 (vanderbilt) IST12348B9 (tigm) IST11563B5 (tigm)
IST14812A6 (tigm) IST11262D9 (tigm) IST14821D3 (tigm) IST14849E7 (tigm)
IST15084G6 (tigm) IST14849E7 (tigm) IST12955G6 (tigm) IST12348B9 (tigm)
IST10151D5 (tigm) IST15069E4 (tigm) IST10463E5 (tigm) IST13731H8 (tigm)
IST12809A11 (tigm) IST14386D11 (tigm) IST11563B5 (tigm)
Private Clones OST471925 (lexicon) OST469148 (lexicon) OST464481 (lexicon) OST461971 (lexicon)
OST459189 (lexicon) OST456262 (lexicon) OST451645 (lexicon) OST450554 (lexicon)
OST447017 (lexicon) OST445482 (lexicon) OST443371 (lexicon) OST442609 (lexicon)
OST441896 (lexicon) OST441331 (lexicon) OST441100 (lexicon) OST440356 (lexicon)
OST439390 (lexicon) OST439107 (lexicon) OST437389 (lexicon) OST437100 (lexicon)
OST435784 (lexicon) OST435738 (lexicon) OST435634 (lexicon) OST435146 (lexicon)
OST431238 (lexicon) OST431122 (lexicon) OST430992 (lexicon) OST426844 (lexicon)
OST426589 (lexicon) OST426517 (lexicon) OST424689 (lexicon) OST424359 (lexicon)
OST420936 (lexicon) OST420829 (lexicon) OST420790 (lexicon) OST420767 (lexicon)
OST420754 (lexicon) OST420737 (lexicon) OST420615 (lexicon) OST420584 (lexicon)
OST420463 (lexicon) OST420388 (lexicon) OST420377 (lexicon) OST420316 (lexicon)
OST420292 (lexicon) OST420286 (lexicon) OST420285 (lexicon) OST417178 (lexicon)
OST417073 (lexicon) OST417006 (lexicon) OST414293 (lexicon) OST414259 (lexicon)
OST414084 (lexicon) OST413957 (lexicon) OST413903 (lexicon) OST413878 (lexicon)
OST413862 (lexicon) OST413603 (lexicon) OST412898 (lexicon) OST412843 (lexicon)
OST412808 (lexicon) OST411320 (lexicon) OST411277 (lexicon) OST411262 (lexicon)
OST411261 (lexicon) OST411250 (lexicon) OST411197 (lexicon) OST411145 (lexicon)
OST409750 (lexicon) OST408844 (lexicon) OST407580 (lexicon) OST407292 (lexicon)
OST407068 (lexicon) OST406619 (lexicon) OST406526 (lexicon) OST406498 (lexicon)
OST406454 (lexicon) OST405731 (lexicon) OST405497 (lexicon) OST405481 (lexicon)
OST405253 (lexicon) OST405247 (lexicon) OST405244 (lexicon) OST405100 (lexicon)
OST404732 (lexicon) OST404692 (lexicon) OST404668 (lexicon) OST404607 (lexicon)
OST404534 (lexicon) OST404397 (lexicon) OST404221 (lexicon) OST404179 (lexicon)
OST404175 (lexicon) OST404143 (lexicon) OST404050 (lexicon) OST403719 (lexicon)
OST403623 (lexicon) OST403507 (lexicon) OST403364 (lexicon) OST402493 (lexicon)
OST402173 (lexicon) OST401961 (lexicon) OST399862 (lexicon) OST399043 (lexicon)
OST395642 (lexicon) OST384057 (lexicon) OST377959 (lexicon) OST367266 (lexicon)
OST366953 (lexicon) OST363869 (lexicon) OST363532 (lexicon) OST362930 (lexicon)
OST358158 (lexicon) OST348772 (lexicon) OST343142 (lexicon) OST341049 (lexicon)
OST335727 (lexicon) OST332272 (lexicon) OST332061 (lexicon) OST330324 (lexicon)
OST326481 (lexicon) OST323087 (lexicon) OST321768 (lexicon) OST316248 (lexicon)
OST306420 (lexicon) OST296173 (lexicon) OST295322 (lexicon) OST282642 (lexicon)
OST272663 (lexicon) OST270356 (lexicon) OST266988 (lexicon) OST263462 (lexicon)
OST263460 (lexicon) OST262697 (lexicon) OST252287 (lexicon) OST249640 (lexicon)
OST235805 (lexicon) OST233849 (lexicon) OST232075 (lexicon) OST221606 (lexicon)
OST198810 (lexicon) OST195164 (lexicon) OST190664 (lexicon) OST183749 (lexicon)
OST180432 (lexicon) OST165237 (lexicon) OST148855 (lexicon) OST144564 (lexicon)
OST139007 (lexicon) OST118275 (lexicon) OST103156 (lexicon) OST101076 (lexicon)
OST101033 (lexicon) OST98343 (lexicon) OST70483 (lexicon) OST67350 (lexicon)
OST66488 (lexicon) OST63383 (lexicon) OST59948 (lexicon) OST59703 (lexicon)
OST59480 (lexicon) OST59479 (lexicon) OST58814 (lexicon) OST58737 (lexicon)
OST58736 (lexicon) OST55549 (lexicon) OST49890 (lexicon) OST48289 (lexicon)
OST46638 (lexicon) OST46361 (lexicon) OST43808 (lexicon) OST43190 (lexicon)
OST41022 (lexicon) OST40051 (lexicon) OST38532 (lexicon) OST38247 (lexicon)
OST38215 (lexicon) OST38112 (lexicon) OST38054 (lexicon) OST37971 (lexicon)
OST37570 (lexicon) OST36311 (lexicon) OST36310 (lexicon) OST35524 (lexicon)
OST34923 (lexicon) OST32955 (lexicon) OST31571 (lexicon) OST28338 (lexicon)
OST27783 (lexicon) OST27368 (lexicon) OST26920 (lexicon) OST24272 (lexicon)
OST23917 (lexicon) OST23839 (lexicon) OST23816 (lexicon) OST23311 (lexicon)
OST23171 (lexicon) OST22294 (lexicon) OST22043 (lexicon) OST20674 (lexicon)
OST15330 (lexicon) OST8766 (lexicon) OST7686 (lexicon) OST6750 (lexicon)
OST2368 (lexicon) OST1153 (lexicon)
Severity of mutation (?) Insertion after 75% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000214916 (Chr8:114466402..114466574 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
TCTCGGGCTCTCAGATTGTT Chr8:114466418..114466437 59.95 50 TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000214916 (Chr8:114466402..114466574 +)
Downstram Exon
ENSMUSE00000397727 (Chr8:114476215..114476814 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
TCTCGGGCTCTCAGATTGTT Chr8:114466418..114466437 59.95 50 GTGTTCTCTCCGCTGTAGGC Chr8:114476295..114476314 60.02 60

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000359315 Chr8:114464622..114464752 GTTGTTGTTGTGGTCGCTTC Chr8:114464652..114464671 59.19 50
upstream ENSMUSE00000214915 Chr8:114465060..114465115 GAAGTACCCCGACCGAGTTC Chr8:114465094..114465113 60.88 60
upstream ENSMUSE00000214916 Chr8:114466402..114466574 TCTCGGGCTCTCAGATTGTT Chr8:114466418..114466437 59.95 50

*** Putative Vector Insertion (Chr 8: 114466575 - 114476214) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000397727 Chr8:114476215..114476814 GTGTTCTCTCCGCTGTAGGC Chr8:114476295..114476314 60.02 60

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GAGGTTCTTCGGAAGCTTGA Chr8:114475536..114475556 59.55 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GAGGTTCTTCGGAAGCTTGA Chr8:114475536..114475556 59.55 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000031950