Tools for the biologist enabling optimized use of gene trap clones
Homepage
|
Blast Search
|
GO Search
|
Advanced Search
|
About
UniTrap UNI12914
Trapped Gene
Cacng7 (
ENSMUSG00000069806
)
Vector Insertion
Chr 7: 3339127 - 3365535
Public Clones
XC212 (baygenomics)
5SD151E03 (ggtc)
3SE035B05 (ggtc)
5SD159B05 (ggtc)
5SE107B11 (ggtc)
(cmhd)
(cmhd)
CMHD-GT_469B8-3 (cmhd)
(cmhd)
(cmhd)
CMHD-GT_355F5-3 (cmhd)
(cmhd)
PST768-1 (escells)
PST21320-NR (escells)
IST12819E7BBF1 (tigm)
IST12065C6 (tigm)
IST12448F8 (tigm)
IST12448F8 (tigm)
IST11182E6 (tigm)
IST12393D5 (tigm)
IST13132B9 (tigm)
IST10605F5 (tigm)
IST14241B5 (tigm)
IST14543D8 (tigm)
IST11073C11 (tigm)
IST11779F5 (tigm)
IST13965E3 (tigm)
IST11073C11 (tigm)
IST12065C6 (tigm)
IST10024C5 (tigm)
IST10011A3 (tigm)
IST11396G10 (tigm)
IST12393D5 (tigm)
IST10184A1 (tigm)
IST12397C5 (tigm)
Private Clones
OST300327 (lexicon)
OST275441 (lexicon)
OST249410 (lexicon)
OST197641 (lexicon)
OST196984 (lexicon)
OST182444 (lexicon)
OST93043 (lexicon)
Severity of mutation
(?)
Insertion after 52% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000677465 (Chr7:3338986..3339126 +)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
ACCATTCTTGCGTTCGTTTC
Chr7:3339084..3339103
60.12
45
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000677465 (Chr7:3338986..3339126 +)
Downstram Exon
ENSMUSE00000578335 (Chr7:3365536..3365681 +)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
ACCATTCTTGCGTTCGTTTC
Chr7:3339084..3339103
60.12
45
GTTCATGACCTCGTCGTTGA
Chr7:3365600..3365619
59.68
50
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000677469
Chr7:3333055..3333136
No primer for this exon
upstream
ENSMUSE00000677467
Chr7:3336656..3336880
AGACCACCGAGGTCAAGATG
Chr7:3336821..3336840
60.11
55
upstream
ENSMUSE00000677466
Chr7:3338545..3338631
CAACTTGGTGACGGAAAACA
Chr7:3338597..3338616
59.58
45
upstream
ENSMUSE00000677465
Chr7:3338986..3339126
ACCATTCTTGCGTTCGTTTC
Chr7:3339084..3339103
60.12
45
*** Putative Vector Insertion (Chr 7: 3339127 - 3365535) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000578335
Chr7:3365536..3365681
GTTCATGACCTCGTCGTTGA
Chr7:3365600..3365619
59.68
50
downstream
ENSMUSE00000578334
Chr7:3366280..3367810
GAGATGGTCCGGGTACTTGA
Chr7:3366516..3366535
59.93
55
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
CGTTCGTTTCTGGCATCTTT
Chr7:3363095..3363115
60.25
45
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
ACTAACACTCCCTGCGTCGT
Chr7:3363161..3363181
59.79
55
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000069806
