Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI12938
Trapped Gene
Jup (ENSMUSG00000001552)
Vector Insertion
Chr 11: 100238313 - 100239402
Public Clones CMHD-GT_350D6-3 (cmhd) IST14316C11 (tigm) IST10010A2 (tigm) IST11733A6 (tigm)
IST13137F12 (tigm) IST11734H6 (tigm) IST11245B4 (tigm) IST13492A2 (tigm)
IST12904B12 (tigm) IST13204E3 (tigm) IST11245B4 (tigm)
Private Clones OST97628 (lexicon)
Severity of mutation (?) Insertion after 67% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000112635 (Chr11:100239403..100239741 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000112635 (Chr11:100239403..100239741 -)
Downstram Exon
ENSMUSE00000112639 (Chr11:100238037..100238312 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000673005 Chr11:100258950..100259077 No primer for this exon
upstream ENSMUSE00000337989 Chr11:100258935..100259063 No primer for this exon
upstream ENSMUSE00000383613 Chr11:100247487..100247702 No primer for this exon
upstream ENSMUSE00000673003 Chr11:100247487..100247699 No primer for this exon
upstream ENSMUSE00000336301 Chr11:100244709..100244968 No primer for this exon
upstream ENSMUSE00000112636 Chr11:100244321..100244559 No primer for this exon
upstream ENSMUSE00000112631 Chr11:100242990..100243191 No primer for this exon
upstream ENSMUSE00000577245 Chr11:100241858..100242002 No primer for this exon
upstream ENSMUSE00000112641 Chr11:100240836..100240939 No primer for this exon
upstream ENSMUSE00000112635 Chr11:100239403..100239741 No primer for this exon

*** Putative Vector Insertion (Chr 11: 100238313 - 100239402) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000112639 Chr11:100238037..100238312 No primer for this exon
downstream ENSMUSE00000112640 Chr11:100235640..100235790 No primer for this exon
downstream ENSMUSE00000112638 Chr11:100235392..100235513 No primer for this exon
downstream ENSMUSE00000112642 Chr11:100233970..100234009 No primer for this exon
downstream ENSMUSE00000400222 Chr11:100233610..100233775 No primer for this exon
downstream ENSMUSE00000360024 Chr11:100231935..100232763 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TACCCAGTAAAGGCCACAGG Chr11:100239380..100239400 59.99 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TACCCAGTAAAGGCCACAGG Chr11:100239380..100239400 59.99 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 CCTGGAGAGTGTGCTGAAGA Chr11:100239715..100239735 59.12 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 CCTGGAGAGTGTGCTGAAGA Chr11:100239715..100239735 59.12 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000001552