Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI13155
Trapped Gene
Ddx19a (ENSMUSG00000015023)
Vector Insertion
Chr 8: 113513510 - 113514376
Public Clones CMHD-GT_324H1-3 (cmhd)
Private Clones OST171035 (lexicon)
Severity of mutation (?) Insertion after 11% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000309896 (Chr8:113514377..113514427 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000309896 (Chr8:113514377..113514427 -)
Downstram Exon
ENSMUSE00000383577 (Chr8:113513374..113513509 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000401459 Chr8:113521567..113521701 No primer for this exon
upstream ENSMUSE00000356437 Chr8:113516957..113517005 No primer for this exon
upstream ENSMUSE00000309896 Chr8:113514377..113514427 No primer for this exon

*** Putative Vector Insertion (Chr 8: 113513510 - 113514376) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000383577 Chr8:113513374..113513509 No primer for this exon
downstream ENSMUSE00000346039 Chr8:113507481..113507573 No primer for this exon
downstream ENSMUSE00000212646 Chr8:113504891..113504993 No primer for this exon
downstream ENSMUSE00000212643 Chr8:113504453..113504567 No primer for this exon
downstream ENSMUSE00000212645 Chr8:113502936..113503113 No primer for this exon
downstream ENSMUSE00000212655 Chr8:113502368..113502605 No primer for this exon
downstream ENSMUSE00000409047 Chr8:113500868..113501030 No primer for this exon
downstream ENSMUSE00000212651 Chr8:113500329..113500520 No primer for this exon
downstream ENSMUSE00000357769 Chr8:113498908..113499990 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GCTAGAGGCTAATCGCCTTG Chr8:113514314..113514334 59.23 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GCACCACTGCAGAAAAGACA Chr8:113514391..113514411 60.03 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000015023