Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI13175
Trapped Gene
Nudcd2 (ENSMUSG00000020328)
Vector Insertion
Chr 11: 40549569 - 40549994
Public Clones D122B10 (ggtc) D122B10 (ggtc) CMHD-GT_476G5-3 (cmhd) (cmhd)
CMHD-GT_325G12-3 (cmhd) CMHD-GT_302B2-3 (cmhd)
Private Clones OST412249 (lexicon) OST202202 (lexicon)
Severity of mutation (?) Insertion after 51% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000103419 (Chr11:40549520..40549568 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000103419 (Chr11:40549520..40549568 +)
Downstram Exon
ENSMUSE00000103421 (Chr11:40549995..40550146 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000350329 Chr11:40547386..40547656 No primer for this exon
upstream ENSMUSE00000103419 Chr11:40549520..40549568 No primer for this exon

*** Putative Vector Insertion (Chr 11: 40549569 - 40549994) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000103421 Chr11:40549995..40550146 No primer for this exon
downstream ENSMUSE00000103424 Chr11:40552661..40553546 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
  No PCR available

Come back to gene ENSMUSG00000020328