Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI13181
Trapped Gene
Ifi35 (ENSMUSG00000010358)
Vector Insertion
Chr 11: 101309937 - 101317879
Public Clones (ggtc) (ggtc) CMHD-GT_325G11-3 (cmhd) CMHD-GT_364A12-3 (cmhd) (cmhd)
Private Clones OST408675 (lexicon) OST390494 (lexicon) OST260110 (lexicon) OST175965 (lexicon)
OST77733 (lexicon) OST56664 (lexicon)
Severity of mutation (?) Insertion after 2% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000585851 (Chr11:101309795..101309936 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000585851 (Chr11:101309795..101309936 +)
Downstram Exon
ENSMUSE00000585850 (Chr11:101317880..101317978 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000585851 Chr11:101309795..101309936 No primer for this exon

*** Putative Vector Insertion (Chr 11: 101309937 - 101317879) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000585850 Chr11:101317880..101317978 No primer for this exon
downstream ENSMUSE00000113027 Chr11:101318504..101318651 No primer for this exon
downstream ENSMUSE00000113024 Chr11:101318730..101318836 No primer for this exon
downstream ENSMUSE00000113025 Chr11:101318934..101319120 No primer for this exon
downstream ENSMUSE00000113023 Chr11:101319205..101319311 No primer for this exon
downstream ENSMUSE00000240598 Chr11:101319515..101319776 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GTGAGGGGCTCCTATCCTGT Chr11:101309945..101309965 60.48 60 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GTGAGGGGCTCCTATCCTGT Chr11:101309945..101309965 60.48 60 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000010358