Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI134
Trapped Gene
Ppp1cc (ENSMUSG00000004455)
Vector Insertion
Chr 5: 122608625 - 122618183
Public Clones GC0086 (tigem) (sanger) (sanger) YTC281 (baygenomics) P032E10 (ggtc)
P137H12 (ggtc) W247A01 (ggtc) D144F10 (ggtc) P138H12 (ggtc) P015D04 (ggtc)
E055E11 (ggtc) D006D02 (ggtc) P137H12 (ggtc) P137H12 (ggtc) P012E12 (ggtc)
D144F10 (ggtc) 3SE307G04 (ggtc) D006D02 (ggtc) CMHD-GT_504G5-3 (cmhd) PST5533-NR (escells)
PST7999-NR (escells) IST14792G5 (tigm) IST10441A1 (tigm) IST10469E2 (tigm)
IST10441A2 (tigm)
Private Clones OST288864 (lexicon) OST266369 (lexicon) OST261296 (lexicon) OST252619 (lexicon)
OST252150 (lexicon) OST222107 (lexicon) OST214493 (lexicon) OST168855 (lexicon)
OST68162 (lexicon) OST67698 (lexicon)
Severity of mutation (?) Insertion after 6% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000706655 (Chr5:122608365..122608624 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000706655 (Chr5:122608365..122608624 +)
Downstram Exon
ENSMUSE00000189074 (Chr5:122618184..122618315 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000706655 Chr5:122608365..122608624 No primer for this exon

*** Putative Vector Insertion (Chr 5: 122608625 - 122618183) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000189074 Chr5:122618184..122618315 No primer for this exon
downstream ENSMUSE00000516431 Chr5:122618928..122619158 No primer for this exon
downstream ENSMUSE00000189075 Chr5:122621831..122621935 No primer for this exon
downstream ENSMUSE00000189069 Chr5:122622741..122622964 No primer for this exon
downstream ENSMUSE00000496953 Chr5:122623155..122623289 No primer for this exon
downstream ENSMUSE00000593285 Chr5:122624064..122624124 No primer for this exon
downstream ENSMUSE00000661838 Chr5:122624064..122625276 No primer for this exon
downstream ENSMUSE00000593284 Chr5:122624989..122625059 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CAACATCGACAGCATCATCC Chr5:122614591..122614611 60.08 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GTGACCGTGACTGGGAAAAC Chr5:122614671..122614691 60.41 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000004455