Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI13692
Trapped Gene
Sparc (ENSMUSG00000018593)
Vector Insertion
Chr 11: 55215574 - 55218681
Public Clones CMHD-GT_261C12-3 (cmhd)
Private Clones OST464813 (lexicon) OST331938 (lexicon) OST213627 (lexicon)
Severity of mutation (?) Insertion after 36% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000448017 (Chr11:55218682..55218803 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000448017 (Chr11:55218682..55218803 -)
Downstram Exon
ENSMUSE00000307396 (Chr11:55215453..55215573 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000448061 Chr11:55233290..55233351 No primer for this exon
upstream ENSMUSE00000678286 Chr11:55233290..55233400 No primer for this exon
upstream ENSMUSE00000448048 Chr11:55223430..55223499 No primer for this exon
upstream ENSMUSE00000708374 Chr11:55223430..55223499 No primer for this exon
upstream ENSMUSE00000448030 Chr11:55220722..55220781 No primer for this exon
upstream ENSMUSE00000678285 Chr11:55220722..55220778 No primer for this exon
upstream ENSMUSE00000448024 Chr11:55220012..55220099 No primer for this exon
upstream ENSMUSE00000448017 Chr11:55218682..55218803 No primer for this exon

*** Putative Vector Insertion (Chr 11: 55215574 - 55218681) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000307396 Chr11:55215453..55215573 No primer for this exon
downstream ENSMUSE00000307389 Chr11:55212694..55212827 No primer for this exon
downstream ENSMUSE00000102602 Chr11:55212050..55212198 No primer for this exon
downstream ENSMUSE00000102587 Chr11:55209303..55209451 No primer for this exon
downstream ENSMUSE00000678288 Chr11:55208939..55209115 No primer for this exon
downstream ENSMUSE00000448041 Chr11:55208002..55209115 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TTGGCGAGTTTGAGAAGGTAA Chr11:55218676..55218697 59.87 42.86 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CCATTGGCGAGTTTGAGAAG Chr11:55218680..55218700 60.77 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000018593