Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI13833
Trapped Gene
AC132406.4-201 (ENSMUSG00000074284)
Vector Insertion
Chr 9: 56904946 - 56905075
Public Clones CMHD-GT_222A8-3 (cmhd)
Private Clones OST85948 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000636631 (Chr9:56905076..56905608 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CTCTGAGGACGGGAACTGAG Chr9:56905108..56905127 59.98 60 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000636631 (Chr9:56905076..56905608 -)
Downstram Exon
ENSMUSE00000636635 (Chr9:56904709..56904945 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CTCTGAGGACGGGAACTGAG Chr9:56905108..56905127 59.98 60 GTGCGCTAAAGCCTATCCTG Chr9:56904733..56904752 60 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000636632 Chr9:56919416..56919614 TATGGCCCTACCACTTGGAC Chr9:56919475..56919494 59.81 55
upstream ENSMUSE00000636631 Chr9:56905076..56905608 CTCTGAGGACGGGAACTGAG Chr9:56905108..56905127 59.98 60

*** Putative Vector Insertion (Chr 9: 56904946 - 56905075) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000636635 Chr9:56904709..56904945 GTGCGCTAAAGCCTATCCTG Chr9:56904733..56904752 60 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 AGGGAGCATCGCTTTTCTTC Chr9:56905088..56905108 60.85 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AGGGAGCATCGCTTTTCTTC Chr9:56905088..56905108 60.85 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000074284