Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI1404
Trapped Gene
Qrich1 (ENSMUSG00000006673)
Vector Insertion
Chr 9: 108421158 - 108430919
Public Clones CH0788 (sanger) XT0589 (sanger) CG0109 (sanger) XT0582 (sanger)
CF0389 (sanger) D051D04 (ggtc)
Private Clones OST455516 (lexicon) OST300258 (lexicon) OST275170 (lexicon) OST213110 (lexicon)
OST33108 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000706684 (Chr9:108421034..108421157 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000706684 (Chr9:108421034..108421157 +)
Downstram Exon
ENSMUSE00000634278 (Chr9:108430920..108431249 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000634279 Chr9:108419445..108419571 No primer for this exon
upstream ENSMUSE00000706684 Chr9:108421034..108421157 No primer for this exon

*** Putative Vector Insertion (Chr 9: 108421158 - 108430919) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000634278 Chr9:108430920..108431249 No primer for this exon
downstream ENSMUSE00000529736 Chr9:108435918..108436949 No primer for this exon
downstream ENSMUSE00000221410 Chr9:108444019..108444196 No primer for this exon
downstream ENSMUSE00000221407 Chr9:108444763..108444917 No primer for this exon
downstream ENSMUSE00000221412 Chr9:108447194..108447308 No primer for this exon
downstream ENSMUSE00000690627 Chr9:108447194..108447331 No primer for this exon
downstream ENSMUSE00000221414 Chr9:108458293..108458401 No primer for this exon
downstream ENSMUSE00000221413 Chr9:108458739..108458890 No primer for this exon
downstream ENSMUSE00000241661 Chr9:108459289..108459379 No primer for this exon
downstream ENSMUSE00000583243 Chr9:108461577..108462491 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GAGGCACTTTAATCGCCTTG Chr9:108427200..108427220 59.85 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CTCAGGACAAGCAGCAGACA Chr9:108421119..108421139 60.33 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000006673