Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI1421
Trapped Gene
Wdr51a (ENSMUSG00000023345)
Vector Insertion
Chr 9: 106232348 - 106252012
Public Clones CH0169 (sanger) CMHD-GT_540H8-3 (cmhd) CMHD-GT_158F6-3 (cmhd) CMHD-GT_515A1-3 (cmhd)
CMHD-GT_479D9-3 (cmhd) CMHD-GT_356B12-3 (cmhd) CMHD-GT_540G8-3 (cmhd) CMHD-GT_363E4-3 (cmhd)
CMHD-GT_497A4-3 (cmhd) CMHD-GT_540F8-3 (cmhd) CMHD-GT_169D4-3 (cmhd) CMHD-GT_540G8-5S (cmhd)
IST12301G7 (tigm)
Private Clones OST452450 (lexicon) OST452446 (lexicon) OST373356 (lexicon) OST90962 (lexicon)
OST60487 (lexicon) OST44436 (lexicon) OST44384 (lexicon) OST43119 (lexicon)
OST31682 (lexicon) OST29933 (lexicon) OST29791 (lexicon) OST29747 (lexicon)
OST29723 (lexicon) OST29494 (lexicon) OST28456 (lexicon) OST26445 (lexicon)
OST26292 (lexicon) OST25275 (lexicon) OST24511 (lexicon) OST24038 (lexicon)
OST23557 (lexicon) OST21552 (lexicon) OST20964 (lexicon) OST20619 (lexicon)
OST19893 (lexicon) OST18372 (lexicon) OST18160 (lexicon) OST18107 (lexicon)
OST18055 (lexicon) OST17749 (lexicon) OST17362 (lexicon) OST16899 (lexicon)
OST16761 (lexicon) OST16672 (lexicon) OST15543 (lexicon) OST15414 (lexicon)
OST15051 (lexicon) OST14650 (lexicon) OST14628 (lexicon) OST14511 (lexicon)
OST13871 (lexicon) OST13274 (lexicon) OST13235 (lexicon) OST13183 (lexicon)
OST12970 (lexicon) OST11746 (lexicon) OST11650 (lexicon) OST11114 (lexicon)
OST9927 (lexicon) OST8192 (lexicon) OST7263 (lexicon) OST7065 (lexicon)
OST2528 (lexicon)
Severity of mutation (?) Insertion after 84% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000358598 (Chr9:106232204..106232347 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CCAAAAATGGATTTGCCTGT Chr9:106232204..106232223 59.8 40 TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000358598 (Chr9:106232204..106232347 +)
Downstram Exon
ENSMUSE00000583348 (Chr9:106252013..106252202 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CCAAAAATGGATTTGCCTGT Chr9:106232204..106232223 59.8 40 TGGCGTTCTCTGCATGATTA Chr9:106252108..106252127 60.37 45

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000583361 Chr9:106183643..106183787 AACTGAACTGGCACAACTGC Chr9:106183690..106183709 58.94 50
upstream ENSMUSE00000583360 Chr9:106185183..106185267 ATTTTAAGGGCCACCGAGAT Chr9:106185205..106185224 59.8 45
upstream ENSMUSE00000634568 Chr9:106187212..106187383 GCTCCCGAGACAAGACTGTT Chr9:106187344..106187363 59.46 55
upstream ENSMUSE00000634567 Chr9:106187724..106187903 CATCGCCAGAGATTCCTGTT Chr9:106187845..106187864 60.22 50
upstream ENSMUSE00000634566 Chr9:106189111..106189218 TCATCGTGTCTGCCAGTGAT Chr9:106189131..106189150 60.28 50
upstream ENSMUSE00000634565 Chr9:106190322..106190437 GACAACACGGTGAAGGTGTG Chr9:106190380..106190399 60.05 55
upstream ENSMUSE00000530303 Chr9:106193338..106193410 No primer for this exon
upstream ENSMUSE00000634564 Chr9:106197472..106197605 ACCCATCGGGAAACTACCTC Chr9:106197505..106197524 60.19 55
upstream ENSMUSE00000634563 Chr9:106207596..106207664 AAGAACGGGGGAGTATTTCG Chr9:106207622..106207641 60.31 50
upstream ENSMUSE00000634562 Chr9:106210272..106210364 CCACCCCTAACCAGCTCTTC Chr9:106210335..106210354 61.01 60
upstream ENSMUSE00000358598 Chr9:106232204..106232347 CCAAAAATGGATTTGCCTGT Chr9:106232204..106232223 59.8 40

*** Putative Vector Insertion (Chr 9: 106232348 - 106252012) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000583348 Chr9:106252013..106252202 TGGCGTTCTCTGCATGATTA Chr9:106252108..106252127 60.37 45

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GCATTTTGGTGCAGCTTCTT Chr9:106250341..106250361 60.4 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GCATTTTGGTGCAGCTTCTT Chr9:106250341..106250361 60.4 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000023345