Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI14300
Trapped Gene
Trak1 (ENSMUSG00000032536)
Vector Insertion
Chr 9: 121206952 - 121273118
Public Clones (sanger) (sanger) (sanger) (sanger) (sanger) (sanger)
(sanger) (sanger) (sanger) (sanger) (sanger) (sanger)
(sanger) (sanger) (sanger) (sanger) (sanger) (sanger)
(sanger) (sanger) (sanger) (sanger) D128F05 (ggtc) D088A08 (ggtc)
5SE286D09 (ggtc) (ggtc) D174E03 (ggtc) D114B02 (ggtc) D081H11 (ggtc)
(ggtc) D138H05 (ggtc) D091H06 (ggtc) D067G12 (ggtc) (ggtc)
E129D03 (ggtc) D118G07 (ggtc) D088A08 (ggtc) 5SE320D06 (ggtc) (ggtc)
D153E04 (ggtc) D114B02 (ggtc) D081H11 (ggtc) (ggtc) D129D03 (ggtc)
D088B12 (ggtc) D060D07 (ggtc) (ggtc) D187A11 (ggtc) D118G07 (ggtc)
D086C11 (ggtc) 3SE320D06 (ggtc) (ggtc) D153E04 (ggtc) D091H06 (ggtc)
D078G05 (ggtc) (ggtc) FHCRC-GT-S16-9H1 (fhcrc) FHCRC-GT-S20-4G1 (fhcrc)
(egtc) IST12151G11BBR1 (tigm) IST13115H3 (tigm) IST15110A11 (tigm)
IST10251G8 (tigm) IST14301D9 (tigm) IST15070G12 (tigm) IST12746D7 (tigm)
IST14613B2 (tigm) IST11494D4 (tigm) IST13112C5 (tigm) IST14622B4 (tigm)
IST13080G1 (tigm) IST14444C8 (tigm) IST14565C12 (tigm) IST14347H12 (tigm)
IST14348G5 (tigm) IST12808G10 (tigm) IST14566E7 (tigm) IST15033F7 (tigm)
IST14201E7 (tigm) IST12746D7 (tigm) IST12913H7 (tigm) IST14752A6 (tigm)
IST14614E10 (tigm) IST11494D4 (tigm) IST14117A1 (tigm) IST14876A11 (tigm)
IST14336B11 (tigm) IST11743F12 (tigm) IST14766G11 (tigm) IST11555E4 (tigm)
IST14613A2 (tigm) IST14155G3 (tigm) IST15061D11 (tigm) IST14515A12 (tigm)
IST15061D11 (tigm) IST12847C6 (tigm) IST14718H4 (tigm) IST14623F2 (tigm)
IST14377G9 (tigm) IST14962H4 (tigm) IST14628F5 (tigm) IST14456D11 (tigm)
IST14617E9 (tigm) IST14755H10 (tigm) IST14766C10 (tigm) IST14718H4 (tigm)
IST14182F4 (tigm) IST14989D6 (tigm) IST12590E10 (tigm) IST10579A10 (tigm)
IST13914E4 (tigm) IST14479G10 (tigm) IST14602A2 (tigm) IST12852D5 (tigm)
IST14702A12 (tigm) IST11177E4 (tigm) IST14191D6 (tigm) IST10008C7 (tigm)
IST11522G4 (tigm) IST12149F8 (tigm) IST14587D4 (tigm) IST14994E3 (tigm)
IST12445E1 (tigm) IST12137G1 (tigm) IST10558B4 (tigm) IST12808G10 (tigm)
IST10719B10 (tigm) IST11590F1 (tigm) IST14338H11 (tigm) IST14334A7 (tigm)
IST14374G2 (tigm) IST12776A9 (tigm) IST14864H11 (tigm) IST14176H10 (tigm)
IST14440F4 (tigm) IST13114A1 (tigm) IST10251G8 (tigm) IST10238G6 (tigm)
IST13077B3 (tigm) IST10008C7 (tigm) IST12313E1 (tigm) IST11584H5 (tigm)
IST14989E7 (tigm) IST14503F1 (tigm) IST13114A1 (tigm) IST14633C2 (tigm)
IST10719B10 (tigm) IST12036E6 (tigm) IST11572E5 (tigm) IST13011G10 (tigm)
IST14682G8 (tigm) IST13914E4 (tigm) IST11012B1 (tigm) IST14584E7 (tigm)
IST10056G11 (tigm) IST11680E5 (tigm) IST12384E10 (tigm) IST12238E11 (tigm)
IST11818G10 (tigm) IST14185B2 (tigm) IST14637A11 (tigm) IST10704E6 (tigm)
IST10945A5 (tigm) IST10467G2 (tigm) IST12322D9 (tigm) IST14846D2 (tigm)
IST12244D7 (tigm) IST12313E1 (tigm) IST14149B11 (tigm) IST14320H7 (tigm)
IST14959C7 (tigm) IST14369G6 (tigm) IST13031E12 (tigm) IST14592B3 (tigm)
IST14555H3 (tigm) IST11555E3 (tigm) IST14286B6 (tigm) IST11513A4 (tigm)
IST14755H10 (tigm) IST14240H7 (tigm) IST12149F8 (tigm) IST14685A8 (tigm)
IST14453A1 (tigm) IST10579A10 (tigm) IST12514F2 (tigm) IST14142A12 (tigm)
IST11552F3 (tigm)
Private Clones OST434444 (lexicon) OST386161 (lexicon) OST374094 (lexicon) OST367511 (lexicon)
OST321200 (lexicon) OST310361 (lexicon) OST303534 (lexicon) OST286878 (lexicon)
OST237075 (lexicon) OST222078 (lexicon) OST219164 (lexicon) OST214315 (lexicon)
OST212433 (lexicon) OST194246 (lexicon) OST90872 (lexicon) OST78186 (lexicon)
OST69058 (lexicon) OST68670 (lexicon) OST66805 (lexicon) OST33743 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000633538 (Chr9:121206620..121206951 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CTACGCTGAGCCTCGCAGTC Chr9:121206831..121206850 63.67 65 TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000633538 (Chr9:121206620..121206951 +)
Downstram Exon
ENSMUSE00000633537 (Chr9:121273119..121273417 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CTACGCTGAGCCTCGCAGTC Chr9:121206831..121206850 63.67 65 CCGGGAGATTCAGTGTACCA Chr9:121273163..121273182 60.91 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000633538 Chr9:121206620..121206951 CTACGCTGAGCCTCGCAGTC Chr9:121206831..121206850 63.67 65

*** Putative Vector Insertion (Chr 9: 121206952 - 121273118) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000633537 Chr9:121273119..121273417 CCGGGAGATTCAGTGTACCA Chr9:121273163..121273182 60.91 55
downstream ENSMUSE00000259358 Chr9:121276076..121276309 CACAGCTCTGAGCCTTCCTC Chr9:121276164..121276183 60.28 60
downstream ENSMUSE00000528168 Chr9:121300975..121301169 AGCGTCTCTTCGATCTGCTC Chr9:121301161..121301180 59.86 55
downstream ENSMUSE00000633536 Chr9:121300975..121301169 AGCGTCTCTTCGATCTGCTC Chr9:121301161..121301180 59.86 55
downstream ENSMUSE00000520674 Chr9:121340597..121340673 AGCCTCGTGACAGCATCAAT Chr9:121340666..121340685 60.83 50
downstream ENSMUSE00000220555 Chr9:121349788..121349904 ATGTGCTCCACCTGCTCTTC Chr9:121349897..121349916 60.42 55
downstream ENSMUSE00000220546 Chr9:121351810..121351910 CAGCTCGTCTTTCATGGACA Chr9:121351854..121351873 59.98 50
downstream ENSMUSE00000497415 Chr9:121352744..121352852 GGAGGACTCGTTCCTCTTCA Chr9:121352768..121352787 59.38 55
downstream ENSMUSE00000501964 Chr9:121354933..121355011 CTGCTGCTCCTTCTCCTCATA Chr9:121354985..121355005 59.73 52.38
downstream ENSMUSE00000499273 Chr9:121355808..121355938 TAAGTCCACGATTTGCGACA Chr9:121355923..121355942 60.26 45
downstream ENSMUSE00000510957 Chr9:121357226..121357300 TGCTGGACAAGCTCTTCATT Chr9:121357260..121357279 58.6 45
downstream ENSMUSE00000508239 Chr9:121357940..121358071 CTCCGCGTACTTATCCTCCA Chr9:121357972..121357991 60.23 55
downstream ENSMUSE00000512812 Chr9:121360769..121360845 CGCATAGTTCCCTCGATTTC Chr9:121360806..121360825 59.67 50
downstream ENSMUSE00000507389 Chr9:121362326..121362562 GTTCATGGCTGAGGACTGGT Chr9:121362443..121362462 60.12 55
downstream ENSMUSE00000528159 Chr9:121363397..121363713 ACTCGGAGAAGCGTGAGTGT Chr9:121363638..121363657 60.06 55
downstream ENSMUSE00000633530 Chr9:121363397..121363862 ACTCGGAGAAGCGTGAGTGT Chr9:121363638..121363657 60.06 55
downstream ENSMUSE00000582958 Chr9:121369477..121369695 CTGAATTGGCGTGGAGGTAG Chr9:121369682..121369701 60.65 55
downstream ENSMUSE00000582957 Chr9:121378103..121378205 CGGCAAGTGGTAAAGGTGAA Chr9:121378166..121378185 61.05 50
downstream ENSMUSE00000582956 Chr9:121381355..121384034 CATGGTTGTCGTGGACTCAC Chr9:121381493..121381512 60.01 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GCCTCCTGCTGAACAGAAAA Chr9:121239942..121239962 60.52 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GCCTCCTGCTGAACAGAAAA Chr9:121239942..121239962 60.52 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000032536