Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI14435
Trapped Gene
Foxj2 (ENSMUSG00000003154)
Vector Insertion
Chr 6: 122770989 - 122778174
Public Clones FHCRC-GT-S13-10G1 (fhcrc)
Private Clones not available
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000692292 (Chr6:122770731..122770988 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000692292 (Chr6:122770731..122770988 +)
Downstram Exon
ENSMUSE00000334179 (Chr6:122778175..122778521 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000692293 Chr6:122770529..122770687 No primer for this exon
upstream ENSMUSE00000452488 Chr6:122770710..122770988 No primer for this exon
upstream ENSMUSE00000692292 Chr6:122770731..122770988 No primer for this exon

*** Putative Vector Insertion (Chr 6: 122770989 - 122778174) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000334179 Chr6:122778175..122778521 No primer for this exon
downstream ENSMUSE00000197518 Chr6:122781454..122781528 No primer for this exon
downstream ENSMUSE00000197520 Chr6:122782956..122783024 No primer for this exon
downstream ENSMUSE00000197516 Chr6:122783179..122783319 No primer for this exon
downstream ENSMUSE00000197519 Chr6:122783699..122783894 No primer for this exon
downstream ENSMUSE00000197525 Chr6:122787839..122788222 No primer for this exon
downstream ENSMUSE00000283359 Chr6:122788602..122788703 No primer for this exon
downstream ENSMUSE00000283351 Chr6:122789479..122789688 No primer for this exon
downstream ENSMUSE00000197523 Chr6:122790244..122790342 No primer for this exon
downstream ENSMUSE00000381256 Chr6:122792782..122795359 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 ACCAGACTTAATCGCCTTGC Chr6:122771032..122771052 59.34 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GACTCGTGACTGGGAAAACC Chr6:122771036..122771056 59.56 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000003154