Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI14583
Trapped Gene
Cxcl17 (ENSMUSG00000060188)
Vector Insertion
Chr 7: 26187875 - 26197906
Public Clones FHCRC-GT-S8-11H1 (fhcrc)
Private Clones not available
Severity of mutation (?) Insertion after 21% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000409338 (Chr7:26197721..26197905 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CTGGTGACAATCCACACAGC Chr7:26197825..26197844 60.16 55 TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000409338 (Chr7:26197721..26197905 -)
Downstram Exon
ENSMUSE00000392891 (Chr7:26187876..26187956 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CTGGTGACAATCCACACAGC Chr7:26197825..26197844 60.16 55 CTTCCAAGAGCCACCTCCTA Chr7:26187878..26187897 59.42 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000409338 Chr7:26197721..26197905 CTGGTGACAATCCACACAGC Chr7:26197825..26197844 60.16 55
upstream ENSMUSE00000392891 Chr7:26187876..26187956 CTAGGAGGTGGCTCTTGGAA Chr7:26187901..26187920 59.42 55

*** Putative Vector Insertion (Chr 7: 26187875 - 26197906) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000386794 Chr7:26187167..26187269 CACAGGGACACTGCTTCCTT Chr7:26187176..26187195 60.3 55
downstream ENSMUSE00000338660 Chr7:26185072..26185463 CTTCCTGTGGTGCTTTTGGT Chr7:26185419..26185438 60.15 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TCCCAAGGGTGTTCTTGTGT Chr7:26188902..26188922 60.4 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TCCCAAGGGTGTTCTTGTGT Chr7:26188902..26188922 60.4 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000060188