Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI14668
Trapped Gene
Zfp207 (ENSMUSG00000017421)
Vector Insertion
Chr 11: 80196998 - 80199404
Public Clones FHCRC-GT-S2-1E1 (fhcrc) IST13572E5 (tigm) IST10472C8 (tigm) IST12937H1 (tigm)
IST10559G1 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 3% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000649789 (Chr11:80196833..80196997 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000649789 (Chr11:80196833..80196997 +)
Downstram Exon
ENSMUSE00000108276 (Chr11:80199405..80199531 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000513672 Chr11:80196832..80196997 No primer for this exon
upstream ENSMUSE00000649789 Chr11:80196833..80196997 No primer for this exon

*** Putative Vector Insertion (Chr 11: 80196998 - 80199404) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000108276 Chr11:80199405..80199531 No primer for this exon
downstream ENSMUSE00000108283 Chr11:80201304..80201442 No primer for this exon
downstream ENSMUSE00000108284 Chr11:80202480..80202647 No primer for this exon
downstream ENSMUSE00000108277 Chr11:80202757..80202832 No primer for this exon
downstream ENSMUSE00000108279 Chr11:80203276..80203323 No primer for this exon
downstream ENSMUSE00000108286 Chr11:80204833..80204903 No primer for this exon
downstream ENSMUSE00000108281 Chr11:80205337..80205494 No primer for this exon
downstream ENSMUSE00000108274 Chr11:80206586..80206678 No primer for this exon
downstream ENSMUSE00000675959 Chr11:80206586..80207785 No primer for this exon
downstream ENSMUSE00000282721 Chr11:80207788..80208033 No primer for this exon
downstream ENSMUSE00000282716 Chr11:80208604..80208763 No primer for this exon
downstream ENSMUSE00000675963 Chr11:80208892..80209637 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GCTTCTTGTAATCGCCTTGC Chr11:80197041..80197061 59.99 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 ATGGGTCGCAAGAAGAAGAA Chr11:80196958..80196978 59.81 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000017421