Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI14924
Trapped Gene
Rcor2 (ENSMUSG00000024968)
Vector Insertion
Chr 19: 7344312 - 7344590
Public Clones PST8420-NR (escells) PST1348-NR (escells) PST13844-NR (escells) PST4363-NR (escells)
PST11132-NR (escells) PST1746-NR (escells) PST1233-NR (escells) IST14447E9 (tigm)
Private Clones OST258044 (lexicon) OST220488 (lexicon) OST182090 (lexicon)
Severity of mutation (?) Insertion after 4% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000621981 (Chr19:7344255..7344311 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
AGTAATTCCGGAGTGCAAGC Chr19:7344289..7344308 59.34 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000621981 (Chr19:7344255..7344311 +)
Downstram Exon
ENSMUSE00000239513 (Chr19:7344591..7344671 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
AGTAATTCCGGAGTGCAAGC Chr19:7344289..7344308 59.34 50 AGCATTCCCTTCAGCTCCTT Chr19:7344636..7344655 60.35 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000454289 Chr19:7341894..7341914 No primer for this exon
upstream ENSMUSE00000481800 Chr19:7343022..7343578 CTGCCTCTCGTAGCCTCAGT Chr19:7343328..7343347 59.77 60
upstream ENSMUSE00000711978 Chr19:7343341..7343578 GGGTAGGGTAATGCCCTCAG Chr19:7343442..7343461 60.7 60
upstream ENSMUSE00000713024 Chr19:7343341..7343578 GGGTAGGGTAATGCCCTCAG Chr19:7343442..7343461 60.7 60
upstream ENSMUSE00000621980 Chr19:7344255..7344311 AGTAATTCCGGAGTGCAAGC Chr19:7344289..7344308 59.34 50
upstream ENSMUSE00000621981 Chr19:7344255..7344311 AGTAATTCCGGAGTGCAAGC Chr19:7344289..7344308 59.34 50

*** Putative Vector Insertion (Chr 19: 7344312 - 7344590) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000239513 Chr19:7344591..7344671 AGCATTCCCTTCAGCTCCTT Chr19:7344636..7344655 60.35 50
downstream ENSMUSE00000146719 Chr19:7345044..7345096 ATAGCCATGCTTCTCCTTGG Chr19:7345087..7345106 59.29 50
downstream ENSMUSE00000550726 Chr19:7345188..7345349 AACAGCACCTTGTCCTCCAC Chr19:7345300..7345319 60.16 55
downstream ENSMUSE00000239457 Chr19:7345460..7345584 CCTGCCTGTCCATCACACTA Chr19:7345550..7345569 59.7 55
downstream ENSMUSE00000454506 Chr19:7345683..7345752 GGGTCTCCAGTATCCGGTTC Chr19:7345745..7345764 60.71 60
downstream ENSMUSE00000239443 Chr19:7345829..7346044 ACTCAGGTACATGCCCTTGG Chr19:7345954..7345973 59.99 55
downstream ENSMUSE00000146721 Chr19:7347590..7347667 GGCTGCTATTGGTCTGCTTC Chr19:7347623..7347642 59.99 55
downstream ENSMUSE00000146716 Chr19:7348323..7348380 AGCGGGAGTTGAACTTGGTA Chr19:7348350..7348369 59.73 50
downstream ENSMUSE00000146718 Chr19:7348510..7348739 CTGCAATAGCCCCAAAGTCT Chr19:7348551..7348570 59.34 50
downstream ENSMUSE00000363943 Chr19:7348833..7349714 GGGGTACTGATCGGGGTACT Chr19:7348881..7348900 60.07 60

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TTGGAACCAATTACCAAGCA Chr19:7344271..7344291 59.01 40 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TTGGAACCAATTACCAAGCA Chr19:7344271..7344291 59.01 40 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000024968