Tools for the biologist enabling optimized use of gene trap clones
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About
UniTrap UNI15135
Trapped Gene
Ehmt2 (
ENSMUSG00000013787
)
Vector Insertion
Chr 17: 35042657 - 35042854
Public Clones
PST10232-NR (escells)
PST12338-NR (escells)
PST9425-NR (escells)
PST10801-NL (escells)
Private Clones
not available
Severity of mutation
(?)
Insertion after 34% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000141510 (Chr17:35042555..35042656 +)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
No primer for this exon
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000141510 (Chr17:35042555..35042656 +)
Downstram Exon
ENSMUSE00000284767 (Chr17:35042855..35043003 +)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
No primer for this exon
No primer for this exon
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000464580
Chr17:35035444..35035485
No primer for this exon
upstream
ENSMUSE00000608074
Chr17:35035919..35036198
No primer for this exon
upstream
ENSMUSE00000486447
Chr17:35036132..35036198
No primer for this exon
upstream
ENSMUSE00000336190
Chr17:35036305..35036511
No primer for this exon
upstream
ENSMUSE00000284974
Chr17:35036605..35036861
No primer for this exon
upstream
ENSMUSE00000360678
Chr17:35039959..35040040
No primer for this exon
upstream
ENSMUSE00000284903
Chr17:35040127..35040164
No primer for this exon
upstream
ENSMUSE00000366225
Chr17:35040268..35040423
No primer for this exon
upstream
ENSMUSE00000405553
Chr17:35042074..35042211
No primer for this exon
upstream
ENSMUSE00000284818
Chr17:35042287..35042401
No primer for this exon
upstream
ENSMUSE00000141510
Chr17:35042555..35042656
No primer for this exon
*** Putative Vector Insertion (Chr 17: 35042657 - 35042854) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000284767
Chr17:35042855..35043003
No primer for this exon
downstream
ENSMUSE00000284746
Chr17:35043096..35043239
No primer for this exon
downstream
ENSMUSE00000284718
Chr17:35043322..35043557
No primer for this exon
downstream
ENSMUSE00000141503
Chr17:35043640..35043822
No primer for this exon
downstream
ENSMUSE00000697819
Chr17:35043898..35044010
No primer for this exon
downstream
ENSMUSE00000141522
Chr17:35043928..35044010
No primer for this exon
downstream
ENSMUSE00000141514
Chr17:35044276..35044382
No primer for this exon
downstream
ENSMUSE00000141523
Chr17:35044467..35044589
No primer for this exon
downstream
ENSMUSE00000141506
Chr17:35044688..35044789
No primer for this exon
downstream
ENSMUSE00000141505
Chr17:35045119..35045223
No primer for this exon
downstream
ENSMUSE00000141518
Chr17:35045349..35045503
No primer for this exon
downstream
ENSMUSE00000141520
Chr17:35045583..35045750
No primer for this exon
downstream
ENSMUSE00000545097
Chr17:35047636..35047780
No primer for this exon
downstream
ENSMUSE00000141509
Chr17:35047923..35048000
No primer for this exon
downstream
ENSMUSE00000141498
Chr17:35048308..35048423
No primer for this exon
downstream
ENSMUSE00000141502
Chr17:35048524..35048610
No primer for this exon
downstream
ENSMUSE00000141507
Chr17:35049334..35049412
No primer for this exon
downstream
ENSMUSE00000545083
Chr17:35049579..35049754
No primer for this exon
downstream
ENSMUSE00000608073
Chr17:35050585..35050990
No primer for this exon
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
GTAATCGCCTTGCAGCACAT
Chr17:35042707..35042727
61.2
50
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
CCATGCGTGACTGGGAAAAC
Chr17:35042703..35042723
63.75
55
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000013787
