Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI1517
Trapped Gene
Mbtd1 (ENSMUSG00000059474)
Vector Insertion
Chr 11: 93752703 - 93766466
Public Clones (sanger) CC0634 (sanger) IST10033A4 (tigm) IST10033B4 (tigm) IST12991D11 (tigm)
IST10033B4 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 1% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000501673 (Chr11:93752653..93752702 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CCTCCACTCAGAGATGTGATGA Chr11:93752665..93752686 60.26 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000501673 (Chr11:93752653..93752702 +)
Downstram Exon
ENSMUSE00000674598 (Chr11:93766467..93766672 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CCTCCACTCAGAGATGTGATGA Chr11:93752665..93752686 60.26 50 CAAACATCCCGAACCAGTCT Chr11:93766528..93766547 59.97 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000674600 Chr11:93747166..93747376 CGGAGGCAACATATTGGAAG Chr11:93747240..93747259 60.46 50
upstream ENSMUSE00000674593 Chr11:93747180..93747435 TCATCGACTTGTGGTCTTGG Chr11:93747358..93747377 59.68 50
upstream ENSMUSE00000481276 Chr11:93747531..93747730 GCCCTGATGGAAAACACAAA Chr11:93747704..93747723 60.87 45
upstream ENSMUSE00000509812 Chr11:93747533..93747730 GCCCTGATGGAAAACACAAA Chr11:93747704..93747723 60.87 45
upstream ENSMUSE00000674596 Chr11:93748194..93748387 CACCATCCGTACGTTCTCCT Chr11:93748288..93748307 59.99 55
upstream ENSMUSE00000478298 Chr11:93748336..93748387 No primer for this exon
upstream ENSMUSE00000501673 Chr11:93752653..93752702 CCTCCACTCAGAGATGTGATGA Chr11:93752665..93752686 60.26 50

*** Putative Vector Insertion (Chr 11: 93752703 - 93766466) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000674598 Chr11:93766467..93766672 CAAACATCCCGAACCAGTCT Chr11:93766528..93766547 59.97 50
downstream ENSMUSE00000621827 Chr11:93766473..93766672 CAAACATCCCGAACCAGTCT Chr11:93766528..93766547 59.97 50
downstream ENSMUSE00000648836 Chr11:93766473..93766672 CAAACATCCCGAACCAGTCT Chr11:93766528..93766547 59.97 50
downstream ENSMUSE00000713589 Chr11:93766473..93766672 CAAACATCCCGAACCAGTCT Chr11:93766528..93766547 59.97 50
downstream ENSMUSE00000648833 Chr11:93770118..93770251 ACCATCCCACACATCTCACA Chr11:93770145..93770164 59.8 50
downstream ENSMUSE00000648831 Chr11:93771597..93771720 TGGGCATAGGCAGCTAATTT Chr11:93771673..93771692 59.7 45
downstream ENSMUSE00000648827 Chr11:93773690..93773772 AGCTGAAGCCTTCCACAGAG Chr11:93773716..93773735 59.74 55
downstream ENSMUSE00000648826 Chr11:93782681..93782798 AGATATCACCCCAGCAGGTC Chr11:93782714..93782733 58.97 55
downstream ENSMUSE00000674591 Chr11:93782681..93782789 AGATATCACCCCAGCAGGTC Chr11:93782714..93782733 58.97 55
downstream ENSMUSE00000110602 Chr11:93784460..93784594 GGAGGAACGAGAGGTTTTCC Chr11:93784592..93784611 60.05 55
downstream ENSMUSE00000110594 Chr11:93785105..93785193 GGAAGTGTTTTGGCACCAGTA Chr11:93785176..93785196 60.02 47.62
downstream ENSMUSE00000421092 Chr11:93785739..93785973 GATTAAGGGGCTGTGCATGT Chr11:93785927..93785946 59.96 50
downstream ENSMUSE00000311768 Chr11:93786981..93787036 ATGTCCGTCCTGTTTCTTCG Chr11:93787009..93787028 60.11 50
downstream ENSMUSE00000521440 Chr11:93787536..93787640 TTGAACCATTCTCCACTCTGG Chr11:93787572..93787592 60.1 47.62
downstream ENSMUSE00000494296 Chr11:93790928..93791075 GAGCCATCAATCCCAATCAT Chr11:93790971..93790990 59.72 45
downstream ENSMUSE00000648811 Chr11:93790928..93791592 AGTCGATCATCCTGGCTTTG Chr11:93791313..93791332 60.22 50
downstream ENSMUSE00000648816 Chr11:93793127..93793209 TTTACTGGTGCTGCAATGGA Chr11:93793199..93793218 60.26 45
downstream ENSMUSE00000648823 Chr11:93793127..93793209 TTTACTGGTGCTGCAATGGA Chr11:93793199..93793218 60.26 45
downstream ENSMUSE00000648815 Chr11:93793534..93793768 GAATCCGTGGTTTGGAACAT Chr11:93793557..93793576 59.65 45
downstream ENSMUSE00000648821 Chr11:93793534..93793768 GAATCCGTGGTTTGGAACAT Chr11:93793557..93793576 59.65 45
downstream ENSMUSE00000674597 Chr11:93793534..93794095 TTGGGATTTTACCTGGCAAC Chr11:93793923..93793942 59.8 45
downstream ENSMUSE00000648813 Chr11:93795195..93795272 No primer for this exon
downstream ENSMUSE00000648819 Chr11:93795195..93795272 No primer for this exon
downstream ENSMUSE00000511364 Chr11:93804976..93806615 CAATAAAACGTCAGCGAGCA Chr11:93805748..93805767 60.01 45
downstream ENSMUSE00000519418 Chr11:93805089..93806615 CAATAAAACGTCAGCGAGCA Chr11:93805748..93805767 60.01 45
downstream ENSMUSE00000674599 Chr11:93805089..93808299 CAATAAAACGTCAGCGAGCA Chr11:93805748..93805767 60.01 45
downstream ENSMUSE00000674601 Chr11:93805089..93806615 CAATAAAACGTCAGCGAGCA Chr11:93805748..93805767 60.01 45

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TACATTGCCTTAATCGCCTTG Chr11:93752744..93752765 60.1 42.86 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TATGCGCCTTAAACCTTACG Chr11:93764736..93764756 58.03 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000059474