Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI15246
Trapped Gene
G3bp2 (ENSMUSG00000029405)
Vector Insertion
Chr 5: 92485294 - 92487097
Public Clones (sanger) PST10321-NL (escells) PST1173-2 (escells) PST4899-NL (escells)
PST2966-NR (escells)
Private Clones OST463234 (lexicon)
Severity of mutation (?) Insertion after 64% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000188558 (Chr5:92486994..92487096 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
GGGTTGATGCTAAGCCAGAA Chr5:92487073..92487092 60.21 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000188558 (Chr5:92486994..92487096 -)
Downstram Exon
ENSMUSE00000323118 (Chr5:92485295..92485423 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
GGGTTGATGCTAAGCCAGAA Chr5:92487073..92487092 60.21 50 TTCTCCGGTTGTCAGAGTCA Chr5:92485360..92485379 59.39 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000513954 Chr5:92512555..92512684 No primer for this exon
upstream ENSMUSE00000188561 Chr5:92502186..92502304 AAGCTCCCGAGTATTTGCAC Chr5:92502188..92502207 59.34 50
upstream ENSMUSE00000188551 Chr5:92499383..92499464 TGTTCATGGTGGAGTGGATG Chr5:92499421..92499440 60.38 50
upstream ENSMUSE00000188547 Chr5:92497351..92497524 GTGGACAGCCTGAGAGGAAG Chr5:92497381..92497400 59.99 60
upstream ENSMUSE00000323144 Chr5:92495518..92495608 No primer for this exon
upstream ENSMUSE00000188545 Chr5:92494778..92494880 AGCGCTTACTATGACGCACA Chr5:92494787..92494806 59.66 50
upstream ENSMUSE00000188556 Chr5:92493351..92493531 AGCCTGAGCCAGAATCTGAA Chr5:92493468..92493487 60.1 50
upstream ENSMUSE00000188546 Chr5:92492363..92492461 CCCCATGTTAAAGCACCAGT Chr5:92492370..92492389 59.85 50
upstream ENSMUSE00000188558 Chr5:92486994..92487096 GGGTTGATGCTAAGCCAGAA Chr5:92487073..92487092 60.21 50
upstream ENSMUSE00000323118 Chr5:92485295..92485423 TGACTCTGACAACCGGAGAA Chr5:92485382..92485401 59.39 50

*** Putative Vector Insertion (Chr 5: 92485294 - 92487097) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000323110 Chr5:92484410..92484528 TTGGTATTGATGCGAAGTTCC Chr5:92484470..92484490 59.95 42.86
downstream ENSMUSE00000597268 Chr5:92483540..92484079 GTCACGATCACGCATCATTC Chr5:92483884..92483903 60.09 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 AGGGTTGATGCTAAGCCAGA Chr5:92487072..92487092 59.84 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AGGGTTGATGCTAAGCCAGA Chr5:92487072..92487092 59.84 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 CAAGGCCAGGTAAGCTCAGT Chr5:92486981..92487001 59.5 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 CAAGGCCAGGTAAGCTCAGT Chr5:92486981..92487001 59.5 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000029405